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카테고리
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Chemokines
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CAT.NO
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LGP-20-035
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PRODUCT
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TARC/CCL17, Human (Thymus and Activation Regulated Chemokine/CCL17)
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SIZE
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20ug, 100ug, 500ug
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PRICE
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KRW 285,000, 880,000, 2,569,000
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Technical Parameters
| Species |
9 |
| Accession |
Q92583 |
| GeneID |
6361 |
| Source |
Escherichia coli. |
| Molecular Weight |
Approximately 8.1 kDa, a single non-glycosylated polypeptide chain containing 71 amino acids. |
| Quantity |
20µg/100µg/500µg |
| AA Sequence |
ARGTNVGREC CLEYFKGAIP LRKLKTWYQT SEDCSRDAIV FVTVQGRAIC SDPNNKRVKN AVKYLQSLER S |
| Purity |
> 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 1.0-10 ng/ml. |
| Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in 20mM PB, pH 7.4, 150mM NaCl. |
| Endotoxin |
Less than 1 EU/μg of rHuTARC/CCL17 as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Usage |
This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
| Reference |
1. Loftus BJ, Kim UJ, Sneddon VP, et al. 1999. Genomics, 60: 295-308.
2. Imai T, Yoshida T, Baba M, et al. 1996. J Biol Chem, 271: 21514-21.
3. Jakubzick C, Wen H, Matsukawa A, et al. 2004. Am J Pathol, 165: 1211-21.
4. Shimada Y, Takehara K, Sato S. 2004. J Dermatol Sci, 34: 201-8.
5. Sumiyoshi K, Nakao A, Setoguchi Y, et al. 2003. J Dermatol Sci, 31: 53-8. |
| Background |
Human CCL17 also known as thymus and activation-related chemokine (TARC) is encoded by the CCL17 gene located on the chromosome 16 in humans. It is expressed by thymus cells constitutively and phytohemagglutinin-stimulated peripheral blood mononuclear cells transiently. CCL17 signals through the chemokine receptors CCR4 and CCR8 and displays chemotactic activity for T lymphocytes and some other leukocytes. It plays an important role in skin diseases such as atopic dermatitis, bullous pemphigoid and mycosis fungoides. CCL17 has approximately 24 – 29 % amino acid sequence identity with RANTES, MIP-1α, MIP-1β, MCP-1, MCP-2, MCP-3 and I-309. |
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