Yeast Expression

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Yeast expressionÀº humanized antibody¸¦ Æ÷ÇÔÇÏ¿© post-translational modificationÀÌ ÇÊ¿äÇÑ ¼¼Æ÷ ³»¿Ü ´Ü¹éÁúÀÇ scale-up productionÀÌ °¡´ÉÇÑ °¡Àå °æÁ¦ÀûÀÎ ÁøÇÙ¼¼Æ÷ ´Ü¹éÁú ¹ßÇö ½Ã½ºÅÛÀÔ´Ï´Ù.

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  • Codon optimization ¼­ºñ½º·Î ½ÃÀÛÇÏ¿© À¯ÀüÀÚ ÇÕ¼º ¹× ¼­ºê Ŭ·Î´×À» °ÅÃÄ ´ç»çÀÇ Yeast protein expression systemÀ» »ç¿ëÇÑ ´Ü¹éÁú ¹ßÇö ¹× Á¤Á¦ °úÁ¤À» all-in-one stepÀ¸·Î ÀÌ¿ë°¡´É
  • High quality, High yield ÀçÁ¶ÇÕ ´Ü¹éÁú Á¦°ø
  • °¡Àå Áøº¸µÈ AKTA Á¤Á¦ ½Ã½ºÅÛ ÀÌ¿ë

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  • Codons Optimization

  • Gene synthesis
    Subcloning
    Sub-cloning & Transformation

  • Small scale expression & Purification test

  • Scale-up Production

  • Purification

  • QC

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Gene synthesis / Subcloning
  • À¯ÀüÀÚ codon optimization ¹× Synthesis
  • E.coli Àü¿ë ¹ßÇöº¤ÅÍ ¼±Á¤ ÈÄ subcloning
  • DNA QC
2-3 ÁÖ
Electroporation / PCR evaluation
  • GS115, X33, KM71¡¦
  • 20 cloning¿¡ ´ëÇÑ PCR screening
1 ÁÖ
Small scale protein expression test
  • 10 cloning¿¡ ´ëÇÑ ¹ßÇö Å×½ºÆ®
  • ÃÖÀû positive clone ¼±Á¤
1 ÁÖ
Large scale protein purification/QC & Quantitation
  • ÃÖÀû positive clone ¹è¾çÀ» scale-up
  • ´Ü¹éÁú Á¤Á¦¸¦ ÅëÇØ °í³óµµÀÇ ´Ü¹éÁú È®º¸
  • SDS-PAGE/ Western blot ºÐ¼®¹ýÀ» ÀÌ¿ëÇÑ Á¤Á¦µÈ ´Ü¹éÁúÀ» °ËÃâÇÏ°í ¼øµµ¸¦ ºÐ¼®
  • BCA ºÐ¼®À» ÅëÇÑ Á¤·®
2-4 ÁÖ

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  • Protein functional study
  • In vitro protein binding assay
  • Enzyme preparation
  • Antigen preparation for antibody production
  • Standard antigen for ELISA design
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