Custom Fluorescent / Reporter Cell Line

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(ÁÖ)·çÁ¨¿¡½º¾¾¾ÆÀÌÀÇ ¹ÙÀÌ·¯½º Á¦ÀÛ ¹× ÇüÁúÀüȯ ±â¼úÀ» È°¿ëÇÏ¿© °í°´¿¡°Ô ¸ÂÃãÇü Çü±¤ Human / Mouse cell lineÀ» Á¦ÀÛÇØ µå¸³´Ï´Ù.
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Fluorescent01 À̹ÌÁö

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  • Áö¼ÓÀû, ¿µ±¸Àû Çü±¤´Ü¹éÁú ¹ßÇö
  • In vitro ¹× in vivo ¿¬±¸¿¡ ´Ù¾çÇÏ°Ô Àû¿ë°¡´É
  • ´Ù¾çÇÑ Çü±¤ ¹× ¹ß±¤¿É¼Ç º¸À¯ (µÎ °¡Áö ¿É¼Ç°¡´É: Çü±¤ + ¹ß±¤´Ü¹éÁú)
  • Çü±¤Çö¹Ì°æ, Multi-reader ¹× In vivo imaging device·Î imaging °á°ú È®º¸
  • ¿øÇÏ´Â ¼¼Æ÷¿¡ ¿øÇÏ´Â Çü±¤ ´Ü¹éÁúÀÌ ¹ßÇöµÇµµ·Ï Á¦ÀÛ
  • ÃÖ±Ù Cell cytotoxicity ÃøÁ¤À» À§ÇØ MTT assay ´ëü ¹æ¹ý À¸·Î »ç¿ëµÇ°í ÀÖÀ½.
  • Fluorescent02 À̹ÌÁö

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  • Expression Vector Cloning

    2 weeks

  • Fluorescent Virus Production

    3 weeks

  • Viral Transduction
    / Antibiotic Selection
    / Polyclone Isolation
    / Fluorescence Check

    2 ~ 3 weeks

  • Single-clone Isolation
    / Clone Expansion

    4 ~ 6 weeks

  • QC: Fluorescence Check, Mycoplasma Test

    1 week

  • Report & Delivery

    within 1 week

Çü±¤/¹ß±¤ ¼±ÅÿɼÇ

Protein Name Promoter Wavelength Application Cell type
CFP CMV Ex.456, Em.480 In vitro Poly/Mono
copGFP EF1 Ex.482, Em.502 In vitro / In vivo
turboGFP EF1 Ex.482, Em.502 In vitro / In vivo
nu-turboGFP
(Nuclear Targetting)
CMV Ex.482, Em.502 In vitro
eGFP EF1 Ex.488, Em.507 In vitro / In vivo
YFP CMV Ex.512, Em.529 In vitro
RFP EF1 Ex.558, Em.605 In vitro
DsRed CMV Ex.558, Em.583 In vitro
iRFP713 EF1 Ex.690, Em.713 In vivo
Luciferase CMV - In vitro / In vivo
GFP/iRFP713 Dual CMV/EF1 - In vitro / In vivo
GFP/Luciferase Dual CMV/EF1 - In vitro / In vivo

* ¼¼Æ÷¼ºÀå Ư¼º»ó Monoclone ºÐ¸®°¡ ¾î·Á¿î ¼¼Æ÷ÀÇ °æ¿ì Polyclone pool ¼¼Æ÷ÁÖ·Î Á¦ÀÛµÉ ¼ö ÀÖÀ½.

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Á¦Ç°ÀÇ QC

  • Fluorescence Check: Fluorescent microscopy (»ó½Ã È®ÀÎ), Multireader (Çü±¤°­µµ Á¤·®)
  • Mycoplasma °ËÃâ°á°ú Á¦°ø
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INQUIRY

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