Crystal Structure of CCL21 and Identification of a Sulfotyrosine Binding Site by NMR
Posted by Brian Volkman on 7/14/2023 to Recent Publications
Custom-made 15N-enriched CCL21 protein supplied by Protein Foundry The chemokine CCL21 guides both T cell and metastatic cancer homing to lymph nodes by activating the receptor CCR7. CCL21 is among a handful of chemokines with an unstructured
Protein Foundry’s CXCL4 Used to Identify Antibodies That Cause Heparin-Induced Thrombocytopenia
Posted by Brian Volkman on 6/7/2023 to Recent Publications
Cloned antibodies from patients with heparin-induced thrombocytopenia (HIT) provide new clues to HIT pathogenesis A recent study published in Blood from scientists at @BloodCenterWI and @MayoClinic reveals structural differences between
Cooperative Chemokine Signaling Influencing T Cell Movement from Blood to Tissue
Posted by Mike Dwinell on 5/19/2023 to Recent Publications
Chemokine positioning assists extravasation of pathogenic human T cells
Assays for Measuring Cell Surface __Expression__ of CXCR4
Posted by Brian Volkman on 2/14/2023 to Recent Publications
When a chemokine binds to its G protein-coupled receptor one of the consequences of receptor activation is a decrease in the levels of receptor present on the cell surface due to more rapid internalization. The mechanism of GPCR internalization
CXCL12 potentiates chromatin compaction via ACKR3 activation
Posted by Brian Volkman on 1/6/2023 to Recent Publications
CXCL12 and its receptor CXCR4 have well known roles in bone marrow homing and mobilization of hematopoietic stem cells and neutrophils, but inflammatory neutrophil recruitment is governed by CXCR2 and its
Chemokine CXCL4 key to rare COVID-19 vaccine side effect
Posted by Brian Volkman on 11/29/2021 to Recent Publications
Chemokine CXCL4 key to rare COVID-19 vaccine side effect
Human CXCL10 identified as a risk factor for cardiovascular disease
Posted by Brian Volkman on 8/31/2021 to Recent Publications
Plasma concentrations of inflammatory proteins can serve as biomarkers for cardiovascular disease (CVD). Previous studies had linked the T cell chemokine CXCL10/IP-10 to hypertension and heart failure, but failed to achieve the statistical significance necessary for its use as a biomarker for human disease.
Human CXCL4 (PF4) linked to Covid-19 vaccine blood clots
Posted by Brian Volkman on 7/20/2021 to Application Note
As reported recently in the Wall Street Journal and other publications, the human chemokine CXCL4 (also known as platelet factor 4 or PF4) is the common link between COVID-19 vaccine-induced immune thrombotic thrombocytopenia (VITT) and a more common condition known as heparin-induced thrombocytopenia (HIT).
Origins of Metamorphism in XCL1
Posted by Brian Volkman on 4/6/2021 to Recent Publications
A new TiBS Spotlight features the work of Protein Foundry scientists and collaborators on the evolution of a highly unusual chemokine.
Study reveals evolutionary origins of fold-switching protein
Posted by Brian Volkman on 1/9/2021 to Recent Publications
A shapeshifting immune system protein called XCL1 evolved from a single-shape ancestor hundreds of millions of years ago. Researchers at the Medical College of Wisconsin discovered the molecular basis for how this happened, in a study
Modeling ligand-receptor specificity
Posted by Brian Volkman on 5/13/2020 to Recent Publications
Using a hybrid modeling approach, Fox generated a model for the chemokine XCL1 in complex with its G protein-coupled receptor XCR1 and identified key interaction sites. Changes within a region of XCL1 that determined its binding energy to XCR1 changed the activity of the receptor and, consequently, cell migration
