Lugen SCI
Lugen R&D Service
- Gene SynthesisÀ¯ÀüÀÚ ÇÕ¼º
- Customized Cloning ServiceÄ¿½ºÅÒ Å¬·Î´× Á¦ÀÛ ¼ºñ½º
- Protein Purification´Ü¹éÁúÁ¤Á¦
- Custom Peptide SynthesisÄ¿½ºÅÒ ÆéŸÀ̵å ÇÕ¼º
- Plasmid DNA Prep ServiceºÐÀÚ »ý¹°ÇÐ ¼ºñ½º
- Lentivirus service·»Æ¼¹ÙÀÌ·¯½ºÁ¦ÀÛ
- Stable Cell Line½ºÅ×ºí ¼¿¶óÀÎ Á¦ÀÛ
- OA-EDSS ServiceÅðÇ༺ °üÀýÀç»ý À¯È¿¹°Áú ½ºÅ©¸®´× ¼ºñ½º
Custom Gene Synthesis
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(ÁÖ)·çÁ¨¿¡½º¾¾¾ÆÀ̴ dzºÎÇÑ À¯ÀüÀÚÇÕ¼º °æ·ÂÀÇ Àü¹®°¡·Î ±¸¼ºµÈ ÆÀ, ÃÖ÷´Ü Àåºñ, Á¤±³ÇÑ À¯ÀüÀÚ ¼³°è ¹× ÃÖÀûÈ ¼ÒÇÁÆ®¿þ¾î¸¦ ±¸ÃàÇÑ ÇÕ¼º »ý¹°ÇÐÀÇ ¼±µÎ ÁÖÀڷμ ´Ù¾çÇÑ ±æÀÌÀÇ À¯ÀüÀÚ ¼¿°ú º¹ÀâÇÑ ±¸Á¶ÀÇ À¯ÀüÀÚ¸¦ ÇÕ¼ºÇÒ ¼ö ÀÖ½À´Ï´Ù. ¸ðµç DNA ¼¿ (GC/AT-rich sequence, repeated sequence, large sequence, toxic sequence,.µî)À» ÇÕ¼ºÇÏ°í ÇÊ¿äÇÑ ¸ðµç º¤ÅͷΠŬ·Î´×ÇÒ ¼ö ÀÖ½À´Ï´Ù. ÀϹÝÀûÀÎ 3,000bpÀÇ ÀÌÇÏÀÇ À¯ÀüÀÚ´Â 2ÁÖ ¸¸¿¡ ÇÕ¼ºÇÏ¿© (ÁÖ)·çÁ¨¿¡½º¾¾¾ÆÀÌ°¡ ¿©·¯ºÐÀÇ ¼ÒÁßÇÑ ½Ã°£À» ¾Æ²¸µå¸®°Ú½À´Ï´Ù. DNA ¿°±â¼¿À» µðÀÚÀÎÇÏ¿© º¸³»Áֽñ⸸ ÇÏ¸é ¾à¼ÓµÈ ½Ã°£ ³»¿¡ ÁÖ¹®ÇÑ ´ë·Î 100% Á¤È®ÇÏ°Ô ÇÕ¼ºÇÏ¿© Àü´ÞÇØ µå¸³´Ï´Ù.
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- Advanced synthetic technology: Repeated sequence, GC-rich ¶Ç´Â AT-rich sequence, Toxic sequence¸¦ Æ÷ÇÔÇÑ ¸ðµç DNA ¿°±â¼¿ ÇÕ¼º °¡´É.
- Codon optimization ¼ºñ½º ¹«»óÁ¦°ø (À¯Àú ¿äû ½Ã)
- Free cloning: º¸À¯ ÁßÀÎ º¤ÅÍ¿¡ ¹«»óÀ¸·Î Ŭ·Î´×ÇÏ¿© Á¦°ø
- QC: Á¦ÇÑÈ¿¼Ò 󸮰á°ú, Sanger sequencing °á°ú È®ÀÎ ÈÄ 100% µ¿Àϼ¿ º¸Àå
- ºü¸¥ ÇÕ¼º & ÇÕ¸®Àû °¡°Ý
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- Template À¯ÀüÀÚ°¡ ¾ø°Å³ª È®º¸°¡ ¾î·Á¿î À¯ÀüÀÚÀÇ °æ¿ì À¯ÀüÁ¤º¸¸¸À» ÀÌ¿ëÇÏ¿© µ¿ÀÏÇÑ À¯ÀüÀÚ¸¦ ÇÕ¼ºÇÔÀ¸·Î½á ¿øÇÏ´Â plasmid Á¦ÀÛ ÀÛ¾÷¿¡ È°¿ë °¡´ÉÇÔ.
- Codon optimizationÀº Ÿ°Ù ´Ü¹éÁú ¹ßÇöÀ» È¿°úÀûÀ¸·Î ³ô¿©ÁÙ ¼ö ÀÖÀ¸¸ç ´Ü¹éÁú ±â´É Çâ»óÀ» °¡´ÉÇÏ°Ô ÇÔ. ¶ÇÇÑ º» ÀÛ¾÷À» ÅëÇØ Á¶Á÷ ÀûÇÕ¼ºÀ» Áõ°¡½Ãų »Ó¸¸ ¾Æ´Ï¶ó ¹ßÇö¿¡ ÀúÇØ¿ä¼ÒÀÎ 2Â÷ ±¸Á¶ ¹× ¹Ýº¹±¸Á¶ Çü¼º °¡´É¼ºÀ» Á¦°ÅÇÒ ¼ö ÀÖÀ½.
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Sequence Optimization
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Oligo Synthesis
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Gene Assemble
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Cloning into Vector
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Sequencing Verification
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Report & Delivery
Length | Turnaround Time (Business Days) |
Price (KW) |
---|---|---|
¡Â 500bp | 7-10 ÀÏ | 200,000 |
500-3000bp | 12-15 ÀÏ | 450/bp |
3001-5000bp | 17-22 ÀÏ | 500/bp |
5001-6000bp | 19-24 ÀÏ | °ßÀû¹®ÀÇ |
> 6000bp | - | °ßÀû¹®ÀÇ |
DNA sequencing | Bi-Automated Fluorescent DNA Sequencing | |
ÃÖÁ¾ ÇüÅ | 4-5 ug Lyophilized DNA cloned in a Standard Plasmid pUC57/pBlueScript II SK(+) vector | |
Á¦°ø ÀÚ·á | Alignment Data, Raw Sequencing Data, Quality Assurance Data, Vector Map Sequence |
! Codon optimization ¼ºñ½º ÁøÇà ½Ã ´ÙÀ½ »çÇ×À» ¾Ë·ÁÁÖ¼¼¿ä.
- cDNA ORF ¼¿ ¹× peptide ¼¿
- ¹ßÇöÇÏ°íÀÚ ÇÏ´Â ±ÕÁÖ ¹× ¼¼Æ÷ÁÖÀÇ Á¤È®ÇÑ À̸§
- ÀÌ¿ëÇÏ°íÀÚ ÇÏ´Â À¯ÀüÀÚ ¾ç³¡ Á¦ÇÑÈ¿¼Ò À̸§
- À¯ÀüÀÚ ³» ¿øÄ¡ ¾Ê´Â Á¦ÇÑÈ¿¼Ò À̸§
- À¯ÀüÀÚ ³» ¹Ýµå½Ã À¯ÁöÇÏ±æ ¿øÇÏ´Â Á¦ÇÑÈ¿¼Ò À̸§
Expression Plasmid Cloning Service
- À¯ÀüÀÚ ÇÕ¼ºÀ» ÁøÇàÇØÁֽŠ°í°´´ÔÀ» ´ë»óÀ¸·Î ¿øÇϽô ¹ßÇöº¤ÅÍ¿¡ Ãß°¡ ¼ºêŬ·Î´× ¼ºñ½º¸¦ ÁøÇàÇØ µå¸³´Ï´Ù.
- À̸¦ À§ÇØ º»»ç´Â ¿©·¯ ¼÷ÁÖ¿¡¼ ¹ßÇö °¡´ÉÇÑ ´Ù¾çÇÑ º¤Å͸¦ º¸À¯ÇÏ°í ÀÖÀ¸¸ç À¯ÀüÀÚ ÇÕ¼º ¹®ÀÇ ½Ã ÇÔ²² ¿äûÇØ Áֽøé Ãß°¡ ¼ºêŬ·Î´×¿¡ ´ëÇÑ ºÎ´ã¾øÀÌ ¿Ï¼ºµÈ º¤Å͸¦ ¹Þ¾Æº¸½Ç ¼ö ÀÖ½À´Ï´Ù.
Host | Vector Series |
---|---|
Standard Cloning Vector | pUC series, pBlueScript II SK |
Baculovirus/Insect | pFastBac series |
Mammalian | pcDNA, pEGFP, pGL3 series |
Yeast | pPIC series |
Bacterial | pET, pGEX, pMAL series |
* ȨÆäÀÌÁö ¼ºñ½º ¹®ÀǶõ¿¡ ¿øÇÏ´Â º¤Å͸íÀ» ±âÀÔÇØ ÁÖ½Ã¸é µË´Ï´Ù.
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Cat No. LGT-1212, Sensi-ES 2x PCR Master Mix with dye (Taq & Pfu) Cat No. LGT-1111, Taq Polymerase with dNTP Mix Cat No. LGT-1112, Sensi-ES Taq Polymerase with dNTP Mix Cat No. LGM-2201, Sensi 1kbp Plus DNA Ladder Marker 0.1~10 kbp Cat No. LGM-2301, Safe-Green Loading Gel Stain Solution (20,000x)