Lugen SCI
Lugen R&D Service
- Gene SynthesisÀ¯ÀüÀÚ ÇÕ¼º
- Customized Cloning ServiceÄ¿½ºÅÒ Å¬·Î´× Á¦ÀÛ ¼ºñ½º
- Protein Purification´Ü¹éÁúÁ¤Á¦
- Custom Peptide SynthesisÄ¿½ºÅÒ ÆéŸÀ̵å ÇÕ¼º
- Plasmid DNA Prep ServiceºÐÀÚ »ý¹°ÇÐ ¼ºñ½º
- Lentivirus service·»Æ¼¹ÙÀÌ·¯½ºÁ¦ÀÛ
- Stable Cell Line½ºÅ×ºí ¼¿¶óÀÎ Á¦ÀÛ
- OA-EDSS ServiceÅðÇ༺ °üÀýÀç»ý À¯È¿¹°Áú ½ºÅ©¸®´× ¼ºñ½º
Retrovirus Packaging Service
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(ÁÖ)·çÁ¨¿¡½º¾¾¾ÆÀ̸¸ÀÇ °íÀ¯ ÇüÁúÀüȯ ±â¼úÀ» ÀÌ¿ëÇÑ ·¹Æ®·Î¹ÙÀÌ·¯½º »ý»ê ½Ã½ºÅÛÀ̸ç VSV-G ¶Ç´Â Eco pseudo-envelop typeÀ¸·Î °í°´ÀÇ ¿ä±¸¿¡ ¸ÂÃç Á¦ÀÛ °¡´ÉÇÕ´Ï´Ù. ·¹Æ®·Î¹ÙÀÌ·¯½º´Â ·»Æ¼¹ÙÀÌ·¯½ºÃ³·³ ¿µ±¸ÀûÀ¸·Î À¯ÀüÀÚ¸¦ µµÀÔÇÏ´Â ½Ã½ºÅÛÀÌÁö¸¸ ·»Æ¼¹ÙÀÌ·¯½º¿Í ´Þ¸® ºÐ¿ÇÏÁö ¾Ê´Â ¼¼Æ÷´Â °¨¿°½ÃÅ°Áö ¸øÇϴ Ư¡ÀÌ ÀÖ½À´Ï´Ù.
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- MMLV (Moloney murine leukemia virus) ·¹Æ®·Î¹ÙÀÌ·¯½º
- MSCV (Murine stem cell virus) ·¹Æ®·Î¹ÙÀÌ·¯½º
- Human cell line: VSV-G pseudo-envelop type ·¹Æ®·Î¹ÙÀÌ·¯½º ÀÌ¿ë
- Mouse cell line: Eco pseudo-envelop type ·¹Æ®·Î¹ÙÀÌ·¯½º ÀÌ¿ë
- ÇØ¿Ü OEM »ý»êÀÌ ¾Æ´Ñ ±¹³» ·»Æ¼¹ÙÀÌ·¯½º »ý»ê½Ã½ºÅÛ: LMO ¼öÀԽŰí ÀýÂ÷°¡ ÇÊ¿ä¾øÀ½
- High stability lentivirus: ÀÚ»ç Á¦Ç°ÀÎ Lenti-Con¢â Reagent¿Í HIV-Safe¢â Manager¸¦ Á¦Ç° Á¦ÀÛ¿¡ È°¿ëÇÏ¿© Ÿ»çÁ¦Ç° ´ëºñ ³ôÀº ¾ÈÁ¤¼º º¸Àå
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Service Type | Titer[RT-qPCR] | Volume | Price (¿ø) | ¼Ò¿ä±â°£ |
---|---|---|---|---|
Pilot scale | > 1 x 10^7 IFU/ml | 0.25 ml | 400,000 | 2 ~ 3ÁÖ |
Medium scale | > 1 x 10^7 IFU/ml > 1 x 10^8 IFU/ml |
1.0 ml 0.1 ml |
1,000,000 | 2 ~ 3ÁÖ |
Large scale | > 1 x 10^7 IFU/ml > 1 x 10^8 IFU/ml |
10 ml 1.0 ml |
°ßÀû¹®ÀÇ | 2 ~ 3ÁÖ |
Vector cloning (< 2 kb) | Retroviral vector ¹«»óÁ¦°ø | + 2ÁÖ |
* ¸ðµç ·¹Æ®·Î¹ÙÀÌ·¯½º Á¦Ç°±º¿¡´Â °í°´ÀÇ Á¦Ç°»ç¿ë ÆíÀǸ¦ À§ÇØ Transduction EnhancerÀÎ PolybreneÀÌ ¹«»óÁ¦°ø µË´Ï´Ù
* º¤ÅÍŬ·Î´×ºÎÅÍ ¹ÙÀÌ·¯½º Á¦ÀÛ±îÁö All-in-one ¼ºñ½º ÀÌ¿ë ½Ã ÃÖÁ¾ ¹ÙÀÌ·¯½º Á¦Ç° ¿Ü¿¡ Á¦ÀÛµÈ Retroviral transfer vector¿Í Àüü¼¿ÀÌ Æ÷ÇÔµÈ Sequence map ÆÄÀÏÀÌ µ¿½Ã Á¦°øµË´Ï´Ù.
Á¦Ç°ÀÇ QC
- ¹ÙÀÌ·¯½º ³óµµÃøÁ¤: RT-qPCR titration