Lugen SCI
Lugen R&D Service
- Gene SynthesisÀ¯ÀüÀÚ ÇÕ¼º
- Customized Cloning ServiceÄ¿½ºÅÒ Å¬·Î´× Á¦ÀÛ ¼ºñ½º
- Protein Purification´Ü¹éÁúÁ¤Á¦
- Custom Peptide SynthesisÄ¿½ºÅÒ ÆéŸÀ̵å ÇÕ¼º
- Plasmid DNA Prep ServiceºÐÀÚ »ý¹°ÇÐ ¼ºñ½º
- Lentivirus service·»Æ¼¹ÙÀÌ·¯½ºÁ¦ÀÛ
- Stable Cell Line½ºÅ×ºí ¼¿¶óÀÎ Á¦ÀÛ
- OA-EDSS ServiceÅðÇ༺ °üÀýÀç»ý À¯È¿¹°Áú ½ºÅ©¸®´× ¼ºñ½º
Lenti-shRNA/miRNA Starter Kit
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shRNA ¶Ç´Â miRNA¸¦ ÀÌ¿ëÇÑ ¿¬±¸´Â Final candidate¸¦ ã±â±îÁö ´Ù¾çÇÑ Candidate¸¦ Å×½ºÆ® ÇØ°í ¼±º°ÇؾßÇÏ´Â ¹ø°Å·Î¿î ÀÛ¾÷ÀÔ´Ï´Ù. µû¶ó¼ (ÁÖ)·çÁ¨¿¡½º¾¾¾ÆÀÌ´Â ÀÌ·¯ÇÑ ¿¬±¸¸¦ ¼öÇàÇÏ°í ÀÖ´Â °í°´µé¿¡°Ô ÆíÀǸ¦ Á¦°øÇÏ°íÀÚ ÇÕ¸®ÀûÀÎ °¡°ÝÀ¸·Î ½±°Ô Á¢±ÙÇÒ ¼ö ÀÖ´Â ·»Æ¼¹ÙÀÌ·¯½º ±â¹ÝÀÇshRNA/miRNA Starter kit¸¦ Á¦°øÇÏ°í ÀÖ½À´Ï´Ù. Scramble Control ·»Æ¼¹ÙÀÌ·¯½º Æ÷ÇÔ 5Á¾ÀÇ ¹ÙÀÌ·¯½º¸¦ Àú·ÅÇÑ °¡°ÝÀ¸·Î ÀÌ¿ëÇÏ½Ç ¼ö ÀÖ½À´Ï´Ù
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- Lentivirus-mediated shRNA/miRNA Expression System
- óÀ½ shRNA/miRNA ¿¬±¸¸¦ ½ÃµµÇÏ´Â ¿¬±¸ÀÚ¸¦ À§ÇÑ Æ¯º°±¸¼º
- Gene accession number Á¦°ø¸¸À¸·Î Scramble control Æ÷ÇÔ ÃÑ 5Á¾ÀÇ specific shRNA/miRNA ¹ßÇö ·»Æ¼¹ÙÀÌ·¯½º (Pilot scale)¸¦ ÇÕ¸®Àû °¡°ÝÀ¸·Î Á¦°ø
- Constitutive or Inducible shRNA ¹ßÇö ¼±Åð¡´É
- Reporter gene (GFP)°ú Selection maker (PuroR) Áß ¼±Åà °¡´É
- 3¼¼´ë VSV-G pseudo-envelop type ·»Æ¼¹ÙÀÌ·¯½º Á¦°ø
- Àü¹®ÀûÀÎ shRNA candidate screening programÀ» ÀÌ¿ëÇÑ shRNA prediction
- pre-miRNA ¼¿ÀÌ Æ÷ÇÔµÈ 450 ~ 500 bpÀÇ genomic ¼¿À» ¢ß·çÁ¨¿¡½º¾¾¾ÆÀÌ À¯ÀüÀÚÇÕ¼º¹ýÀ¸·Î ÇÕ¼ºÇÏ¿© Á¦°ø
- High Stability Lentivirus: ÀÚ»ç Á¦Ç°ÀÎ Lenti-Con¢â Reagent¿Í HIV-Safe¢â Manager¸¦ Á¦Ç° Á¦ÀÛ¿¡ È°¿ëÇÏ¿© Ÿ»çÁ¦Ç° ´ëºñ ³ôÀº ¾ÈÁ¤¼º º¸Àå
- ÀÚ»çÁ¦Ç°ÀÎ Transduction enhancer, Lenti-TD MAX (100 x, 0.5 ml) ¹«»óÁ¦°ø
- Lentiviral transfer plasmid ¹× Àüü¼¿ ÆÄÀÏ Á¦°ø
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Kit Type | Titer[RT-qPCR] | Volume | Price (¿ø) | ¼Ò¿ä±â°£ |
---|---|---|---|---|
shRNA Starter Kit | > 1 x 10^8 IFU/ml (Scramble control Æ÷ÇÔ 4Á¾ shRNA lentivirus) |
0.25 ml x 5 ea | 3,500,000 | 5 ~ 6ÁÖ |
miRNA Starter Kit | > 1 x 10^8 IFU/ml (Scramble control Æ÷ÇÔ 4Á¾ miRNA lentivirus)) |
0.25 ml x 5 ea | 4,500,000 | À¯ÀüÀÚ ÇÕ¼º ~ 3ÁÖ |
Ŭ·Î´×/¹ÙÀÌ·¯½º 5 ~ 6ÁÖ |
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- Package 1 (-20¡É)
- Lentiviral vector package (4Á¾ lentiviral vector DNA, Full sequence Á¦°ø)
- Transduction enhancer, Lenti-TD MAX (100 x, 0.5 ml)
- Package 2 (-80¡É)
- Lentivirus package (In vitro grade): Scramble virus 1Á¾, Candidate virus 4Á¾, ÃÑ 5Á¾
Á¦Ç°ÀÇ QC
- ¹ÙÀÌ·¯½º ³óµµÃøÁ¤: RT-qPCR titration