Lugen SCI
Lugen R&D Service
- Gene SynthesisÀ¯ÀüÀÚ ÇÕ¼º
- Customized Cloning Serviceº¤ÅÍŬ·Î´× Á¦ÀÛ¼ºñ½º
- Protein Purification´Ü¹éÁúÁ¤Á¦
- Custom Peptide SynthesisÄ¿½ºÅÒ ÆéŸÀ̵å ÇÕ¼º
- Plasmid DNA Prep ServiceºÐÀÚ »ý¹°ÇÐ ¼ºñ½º
- Lentivirus service·»Æ¼¹ÙÀÌ·¯½ºÁ¦ÀÛ
- Stable Cell Line½ºÅ×ºí ¼¿¶óÀÎ Á¦ÀÛ
- OA-EDSS ServiceÅðÇ༺ °üÀýÀç»ý À¯È¿¹°Áú ½ºÅ©¸®´× ¼ºñ½º
Custom Fluorescent / Reporter Cell Line
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(ÁÖ)·çÁ¨¿¡½º¾¾¾ÆÀÌÀÇ ¹ÙÀÌ·¯½º Á¦ÀÛ ¹× ÇüÁúÀüȯ ±â¼úÀ» È°¿ëÇÏ¿© °í°´¿¡°Ô ¸ÂÃãÇü Çü±¤ Human / Mouse cell lineÀ» Á¦ÀÛÇØ µå¸³´Ï´Ù.
¿©·¯ºÐÀÇ º¸À¯ÇÏ°í °è½Å ¼¼Æ÷ÁÖ¸¦ È°¿ëÇÏ¿© ³í¹®ÀÇ ÁúÀ» Çâ»ó½Ãų ¼ö ÀÖ´Â In Vitro / In Vivo Live Cell Imaging °á°ú¸¦ °æÁ¦ÀûÀÎ °¡°ÝÀ¸·Î ¸¸µé¾î º¸½Ã±â ¹Ù¶ø´Ï´Ù
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- Áö¼ÓÀû, ¿µ±¸Àû Çü±¤´Ü¹éÁú ¹ßÇö
- In vitro ¹× in vivo ¿¬±¸¿¡ ´Ù¾çÇÏ°Ô Àû¿ë°¡´É
- ´Ù¾çÇÑ Çü±¤ ¹× ¹ß±¤¿É¼Ç º¸À¯ (µÎ °¡Áö ¿É¼Ç°¡´É: Çü±¤ + ¹ß±¤´Ü¹éÁú)
- Çü±¤Çö¹Ì°æ, Multi-reader ¹× In vivo imaging device·Î imaging °á°ú È®º¸
- ¿øÇÏ´Â ¼¼Æ÷¿¡ ¿øÇÏ´Â Çü±¤ ´Ü¹éÁúÀÌ ¹ßÇöµÇµµ·Ï Á¦ÀÛ
- ÃÖ±Ù Cell cytotoxicity ÃøÁ¤À» À§ÇØ MTT assay ´ëü ¹æ¹ý À¸·Î »ç¿ëµÇ°í ÀÖÀ½.
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-
Expression Vector Cloning
2 weeks -
Fluorescent Virus Production
3 weeks -
Viral Transduction
/ Antibiotic Selection
/ Polyclone Isolation
/ Fluorescence Check
2 ~ 3 weeks -
Single-clone Isolation
/ Clone Expansion
4 ~ 6 weeks -
QC: Fluorescence Check, Mycoplasma Test
1 week -
Report & Delivery
within 1 week
Çü±¤/¹ß±¤ ¼±ÅÿɼÇ
Protein Name | Promoter | Wavelength | Application | Cell type |
---|---|---|---|---|
CFP | CMV | Ex.456, Em.480 | In vitro | Poly/Mono |
copGFP | EF1 | Ex.482, Em.502 | In vitro / In vivo | |
turboGFP | EF1 | Ex.482, Em.502 | In vitro / In vivo | |
nu-turboGFP (Nuclear Targetting) |
CMV | Ex.482, Em.502 | In vitro | |
eGFP | EF1 | Ex.488, Em.507 | In vitro / In vivo | |
YFP | CMV | Ex.512, Em.529 | In vitro | |
RFP | EF1 | Ex.558, Em.605 | In vitro | |
DsRed | CMV | Ex.558, Em.583 | In vitro | |
iRFP713 | EF1 | Ex.690, Em.713 | In vivo | |
Luciferase | CMV | - | In vitro / In vivo | |
GFP/iRFP713 Dual | CMV/EF1 | - | In vitro / In vivo | |
GFP/Luciferase Dual | CMV/EF1 | - | In vitro / In vivo |
* ¼¼Æ÷¼ºÀå Ư¼º»ó Monoclone ºÐ¸®°¡ ¾î·Á¿î ¼¼Æ÷ÀÇ °æ¿ì Polyclone pool ¼¼Æ÷ÁÖ·Î Á¦ÀÛµÉ ¼ö ÀÖÀ½.
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- ȨÆäÀÌÁö ¼ºñ½º °ßÀû¶õ¿¡ ¹®Àǹٶø´Ï´Ù.
Á¦Ç°ÀÇ QC
- Fluorescence Check: Fluorescent microscopy (»ó½Ã È®ÀÎ), Multireader (Çü±¤°µµ Á¤·®)
- Mycoplasma °ËÃâ°á°ú Á¦°ø