Custom Stable Cell Line(Knock-in / Knock-down / Knock-out / Inducible)

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(ÁÖ)·çÁ¨¿¡½º¾¾¾ÆÀÌÀÇ ¹ÙÀÌ·¯½º Á¦ÀÛ ¹× ÇüÁúÀüȯ ±â¼úÀ» È°¿ëÇÏ¿© °í°´¿¡°Ô ¸ÂÃãÇü Human / Mouse cell lineÀ» Á¦ÀÛÇØ µå¸³´Ï´Ù. cell line Á¦ÀÛ¿¡ ¾î·Á¿òÀ» °Þ°í Àְųª ¿¬±¸ ÀÎÇÁ¶ó°¡ ºÎÁ·ÇϽŠ°í°´µé¿¡°Ô ¹ÙÀÌ·¯½º Á¦ÀÛºÎÅÍ cell line Á¦ÀÛ±îÁö ¸ÂÃãÇü all-in one ¼­ºñ½º Á¦°øÀÌ °¡´ÉÇÕ´Ï´Ù.

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  • cDNA °ú¹ßÇö ¼¼Æ÷ÁÖ
  • Knock-down (KD) ¼¼Æ÷ÁÖ
  • Knock-out (KO, CRISPR/Cas9) ¼¼Æ÷ÁÖ
  • Dox À¯µµ¹ßÇö (TetO inducible system) ¼¼Æ÷ÁÖ

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  • StableCellLine À̹ÌÁö
  • ·»Æ¼/·¹Æ®·Î ¹ÙÀÌ·¯½º °¨¿°½Ã½ºÅÛÀ» ÀÌ¿ëÇÑ ¼¼Æ÷ÁÖ »ý»ê
  • Single cell clone Á¦°øÀ¸·Î ±ÕÀÏÇÏ°í ¾ÈÁ¤ÀûÀΠŸ°Ù À¯ÀüÀÚ °ú¹ßÇö/¾ïÁ¦ °¡´É
  • cDNA (KI), shRNA (KD), gRNA (KO) ¹ßÇö, Dox À¯µµ¹ßÇö single cell clone Á¦ÀÛ °¡´É
  • À¯ÀüÀÚ Å¬·Î´×ºÎÅÍ ¼¼Æ÷ÁÖ Á¦ÀÛ, ¹ßÇö È®ÀαîÁö all-in one ¼­ºñ½º Á¦°ø
  • Human (·»Æ¼¹ÙÀÌ·¯½º È°¿ë), Mouse (Eco-·¹Æ®·Î¹ÙÀÌ·¯½º È°¿ë) ¼¼Æ÷ÁÖ ¸ðµÎ °¡´É
  • ¹ßÇö·®ÀÌ ´Ù¾çÇÑ 5 ~ 6Á¾ÀÇ ¼¼Æ÷ÁÖ Á¦°ø, À¯Àú ¿¬±¸¸ñÀû¿¡ ¸Â°Ô ¼±ÅÃÈ°¿ë °¡´É
  • È®¸³µÈ ¸ðµç Ŭ·Ð¿¡ ´ëÇÑ mycoplasma À¯¹« È®ÀÎ ÈÄ Á¦°ø

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  • Expression Vector Cloning

    2 weeks

  • Virus Production

    3 weeks

  • Viral Transduction / Antibiotic Selection / Polyclone Isolation / Expression Test

    2 ~ 3 weeks

  • Single-clone Isolation / Clone Expansion

    4 ~ 6 weeks

  • QC: Expression Test Mycoplasma Test

    1 week

  • Report & Delivery

    within 1 week

* Á¦ÀÛ ¿äûÇϽŠ¼¼Æ÷ÁÖÀÇ °íÀ¯ ¼ºÀå·ü¿¡ µû¶ó »ó±â Á¦Á¶±â°£Àº ´Þ¶óÁú ¼ö ÀÖÀ½.

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Á¦Ç°ÀÇ QC

  • Expression Test: RT-qPCR titration ±âº»Á¦°ø (Western blot ºÐ¼®À¸·Î ´ëü °¡´ÉÇϳª °í°´ÀÌ ÀÏÂ÷ Ç×ü Á¦°ø ¿øÄ¢)
  • Mycoplasma °ËÃâ°á°ú Á¦°ø

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  • cDNA ±¸ÀÔ ¹× À¯ÀüÀÚÇÕ¼º °¡°ÝÀº º°µµÀÌ¸ç ±âº» Àç·áÁغñ ±â°£À¸·Î ÃÑ ¼­ºñ½º ÁøÇà±â°£¿¡ Æ÷ÇÔµÇÁö ¾ÊÀ½.
  • º»»ç º¸À¯ ¼¼Æ÷ÁÖÀÇ °æ¿ì º»»çÀÇ ¼¼Æ÷ÁÖ·Î Á¦ÀÛÀ» ÁøÇàÇÔ.
  • °í°´Á¦°ø ¼¼Æ÷ÁÖ·Î Á¦ÀÛ ½Ã º»»ç¿¡ ¼¼Æ÷ÁÖ ÀÔ°í ÈÄ º°µµÀÇ QC °úÁ¤À» °ÅÃÄ¾ß ÇÔ.
  • º»»çÀÇ QC Å×½ºÆ® (Mycoplasma °¨¿°¿©ºÎ È®ÀÎ)¸¦ Åë°úÇؾ߸¸ cell line Á¦Á¶¿¡ »ç¿ë°¡´ÉÇÔ.
  • ¼¼Æ÷ÁÖ QC ÈÄ ºÎÀûÇÕ ÆÇÁ¤ÀÌ ³ª¿À´Â °æ¿ì ¼¼Æ÷ÁÖ¸¦ ´Ù½Ã ±¸ÀÔÇϽðųª ´Ù¸¥ lotÀÇ ¼¼Æ÷ÁÖ¸¦ Á¦°øÇØ¾ß ÇÔ.
  • ¼¼Æ÷ÁÖ Á¦ÀÛ ½Ã °¢ Á¦Á¶´Ü°è¿¡¼­ ƯÁ¤ À¯ÀüÀÚ ¹ßÇö ¹× ¾ïÁ¦ µîÀ¸·Î ÀÎÇØ ¼¼Æ÷ÁÖ ¼ºÀå¿¡ ½É°¢ÇÑ ¹®Á¦°¡ ¹ß»ýÇÒ °æ¿ì Á¦ÀÛ°úÁ¤Àº ÁߴܵǸç ÁøÇàµÈ Àü ´Ü°è±îÁöÀÇ ºñ¿ë¸¸ û±¸µÊ.
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