Custom Gene Synthesis

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(ÁÖ)·çÁ¨¿¡½º¾¾¾ÆÀ̴ dzºÎÇÑ À¯ÀüÀÚÇÕ¼º °æ·ÂÀÇ Àü¹®°¡·Î ±¸¼ºµÈ ÆÀ, ÃÖ÷´Ü Àåºñ, Á¤±³ÇÑ À¯ÀüÀÚ ¼³°è ¹× ÃÖÀûÈ­ ¼ÒÇÁÆ®¿þ¾î¸¦ ±¸ÃàÇÑ ÇÕ¼º »ý¹°ÇÐÀÇ ¼±µÎ ÁÖÀڷμ­ ´Ù¾çÇÑ ±æÀÌÀÇ À¯ÀüÀÚ ¼­¿­°ú º¹ÀâÇÑ ±¸Á¶ÀÇ À¯ÀüÀÚ¸¦ ÇÕ¼ºÇÒ ¼ö ÀÖ½À´Ï´Ù. ¸ðµç DNA ¼­¿­ (GC/AT-rich sequence, repeated sequence, large sequence, toxic sequence,.µî)À» ÇÕ¼ºÇÏ°í ÇÊ¿äÇÑ ¸ðµç º¤ÅͷΠŬ·Î´×ÇÒ ¼ö ÀÖ½À´Ï´Ù. ÀϹÝÀûÀÎ 3,000bpÀÇ ÀÌÇÏÀÇ À¯ÀüÀÚ´Â 2ÁÖ ¸¸¿¡ ÇÕ¼ºÇÏ¿© (ÁÖ)·çÁ¨¿¡½º¾¾¾ÆÀÌ°¡ ¿©·¯ºÐÀÇ ¼ÒÁßÇÑ ½Ã°£À» ¾Æ²¸µå¸®°Ú½À´Ï´Ù. DNA ¿°±â¼­¿­À» µðÀÚÀÎÇÏ¿© º¸³»Áֽñ⸸ ÇÏ¸é ¾à¼ÓµÈ ½Ã°£ ³»¿¡ ÁÖ¹®ÇÑ ´ë·Î 100% Á¤È®ÇÏ°Ô ÇÕ¼ºÇÏ¿© Àü´ÞÇØ µå¸³´Ï´Ù.

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  • Advanced synthetic technology: Repeated sequence, GC-rich ¶Ç´Â AT-rich sequence, Toxic sequence¸¦ Æ÷ÇÔÇÑ ¸ðµç DNA ¿°±â¼­¿­ ÇÕ¼º °¡´É.
  • Codon optimization ¼­ºñ½º ¹«»óÁ¦°ø (À¯Àú ¿äû ½Ã)
  • Free cloning: º¸À¯ ÁßÀÎ º¤ÅÍ¿¡ ¹«»óÀ¸·Î Ŭ·Î´×ÇÏ¿© Á¦°ø
  • QC: Á¦ÇÑÈ¿¼Ò 󸮰á°ú, Sanger sequencing °á°ú È®ÀÎ ÈÄ 100% µ¿Àϼ­¿­ º¸Àå
  • ºü¸¥ ÇÕ¼º & ÇÕ¸®Àû °¡°Ý

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  • Template À¯ÀüÀÚ°¡ ¾ø°Å³ª È®º¸°¡ ¾î·Á¿î À¯ÀüÀÚÀÇ °æ¿ì À¯ÀüÁ¤º¸¸¸À» ÀÌ¿ëÇÏ¿© µ¿ÀÏÇÑ À¯ÀüÀÚ¸¦ ÇÕ¼ºÇÔÀ¸·Î½á ¿øÇÏ´Â plasmid Á¦ÀÛ ÀÛ¾÷¿¡ È°¿ë °¡´ÉÇÔ.
  • Codon optimizationÀº Ÿ°Ù ´Ü¹éÁú ¹ßÇöÀ» È¿°úÀûÀ¸·Î ³ô¿©ÁÙ ¼ö ÀÖÀ¸¸ç ´Ü¹éÁú ±â´É Çâ»óÀ» °¡´ÉÇÏ°Ô ÇÔ. ¶ÇÇÑ º» ÀÛ¾÷À» ÅëÇØ Á¶Á÷ ÀûÇÕ¼ºÀ» Áõ°¡½Ãų »Ó¸¸ ¾Æ´Ï¶ó ¹ßÇö¿¡ ÀúÇØ¿ä¼ÒÀÎ 2Â÷ ±¸Á¶ ¹× ¹Ýº¹±¸Á¶ Çü¼º °¡´É¼ºÀ» Á¦°ÅÇÒ ¼ö ÀÖÀ½.

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  • Sequence Optimization

  • Oligo Synthesis

  • Gene Assemble

  • Cloning into Vector

  • Sequencing Verification

  • Report & Delivery

Length Turnaround Time
(Business Days)
Price (KW)
¡Â 500bp 7-10 ÀÏ 200,000
500-3000bp 12-15 ÀÏ 450/bp
3001-5000bp 17-22 ÀÏ 500/bp
5001-6000bp 19-24 ÀÏ °ßÀû¹®ÀÇ
> 6000bp - °ßÀû¹®ÀÇ
DNA sequencing Bi-Automated Fluorescent DNA Sequencing
ÃÖÁ¾ ÇüÅ 4-5 ug Lyophilized DNA cloned in a Standard Plasmid pUC57/pBlueScript II SK(+) vector
Á¦°ø ÀÚ·á Alignment Data, Raw Sequencing Data, Quality Assurance Data, Vector Map Sequence

! Codon optimization ¼­ºñ½º ÁøÇà ½Ã ´ÙÀ½ »çÇ×À» ¾Ë·ÁÁÖ¼¼¿ä.

  1. cDNA ORF ¼­¿­ ¹× peptide ¼­¿­
  2. ¹ßÇöÇÏ°íÀÚ ÇÏ´Â ±ÕÁÖ ¹× ¼¼Æ÷ÁÖÀÇ Á¤È®ÇÑ À̸§
  3. ÀÌ¿ëÇÏ°íÀÚ ÇÏ´Â À¯ÀüÀÚ ¾ç³¡ Á¦ÇÑÈ¿¼Ò À̸§
  4. À¯ÀüÀÚ ³» ¿øÄ¡ ¾Ê´Â Á¦ÇÑÈ¿¼Ò À̸§
  5. À¯ÀüÀÚ ³» ¹Ýµå½Ã À¯ÁöÇÏ±æ ¿øÇÏ´Â Á¦ÇÑÈ¿¼Ò À̸§

Expression Plasmid Cloning Service

  • À¯ÀüÀÚ ÇÕ¼ºÀ» ÁøÇàÇØÁֽŠ°í°´´ÔÀ» ´ë»óÀ¸·Î ¿øÇϽô ¹ßÇöº¤ÅÍ¿¡ Ãß°¡ ¼­ºêŬ·Î´× ¼­ºñ½º¸¦ ÁøÇàÇØ µå¸³´Ï´Ù.
  • À̸¦ À§ÇØ º»»ç´Â ¿©·¯ ¼÷ÁÖ¿¡¼­ ¹ßÇö °¡´ÉÇÑ ´Ù¾çÇÑ º¤Å͸¦ º¸À¯ÇÏ°í ÀÖÀ¸¸ç À¯ÀüÀÚ ÇÕ¼º ¹®ÀÇ ½Ã ÇÔ²² ¿äûÇØ Áֽøé Ãß°¡ ¼­ºêŬ·Î´×¿¡ ´ëÇÑ ºÎ´ã¾øÀÌ ¿Ï¼ºµÈ º¤Å͸¦ ¹Þ¾Æº¸½Ç ¼ö ÀÖ½À´Ï´Ù.
Host Vector Series
Standard Cloning Vector pUC series, pBlueScript II SK
Baculovirus/Insect pFastBac series
Mammalian pcDNA, pEGFP, pGL3 series
Yeast pPIC series
Bacterial pET, pGEX, pMAL series

* ȨÆäÀÌÁö ¼­ºñ½º ¹®ÀǶõ¿¡ ¿øÇÏ´Â º¤Å͸íÀ» ±âÀÔÇØ ÁÖ½Ã¸é µË´Ï´Ù.

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INQUIRY

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