Lugen SCI
Lugen R&D Service
- Gene SynthesisÀ¯ÀüÀÚ ÇÕ¼º
- Customized Cloning ServiceÄ¿½ºÅÒ Å¬·Î´× Á¦ÀÛ ¼ºñ½º
- Protein Purification´Ü¹éÁúÁ¤Á¦
- Custom Peptide SynthesisÄ¿½ºÅÒ ÆéŸÀ̵å ÇÕ¼º
- Plasmid DNA Prep ServiceºÐÀÚ »ý¹°ÇÐ ¼ºñ½º
- Lentivirus service·»Æ¼¹ÙÀÌ·¯½ºÁ¦ÀÛ
- Stable Cell Line½ºÅ×ºí ¼¿¶óÀÎ Á¦ÀÛ
- OA-EDSS ServiceÅðÇ༺ °üÀýÀç»ý À¯È¿¹°Áú ½ºÅ©¸®´× ¼ºñ½º
Western Blot Analysis Service
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ÀÌ·¯ÇÑ ³ëÇϿ츦 ¹ÙÅÁÀ¸·Î °í°´ º¸À¯ ´Ü¹éÁú »ùÇÿ¡ ´ëÇÑ Western blotting ½ÇÇè´ëÇà ºÐ¼®¼ºñ½º¸¦ Á¦°øÇÏ°í ÀÖ½À´Ï´Ù.
Western blot ºÐ¼®¹ýÀº ´Ü¹éÁúÀ» SDS-PAGE Àü±â¿µµ¿ ÈÄ, nitrocellulose³ª PVDF membrane¿¡ °íÁ¤½ÃÄÑ
Ç׿ø-Ç×ü ¹ÝÀÀÀ» ÅëÇØ ¸ñÀû ´Ü¹éÁú¸¸À» °ËÃâÇÏ´Â classic protein assay Áß ÇϳªÀÔ´Ï´Ù.
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- Tag (His-, HA-, Flag-. GFP) fusion ´Ü¹éÁú °ËÃ⠽à ´ç»ç¿¡¼ ÀÏÂ÷Ç×ü Á¦°ø
- ƯÀÌ ÀÏÂ÷Ç×ü´Â °í°´ÀÌ Á¦°ø¿øÄ¢ (´ç»ç º¸À¯ ÁßÀΠƯÀÌ ÀÏÂ÷Ç×ü´Â Á¦°ø°¡´É: Ãß°¡ charge ÀÖÀ½)
- ¸ðµç ÀÌÂ÷Ç×ü´Â ´ç»ç º¸À¯ Ç×ü·Î ÁøÇà°¡´É
- ±âº»ºÐ¼® »ùÇà ¼ö: 7 samples / one blot
- ´ç»ç Á¦Ç°ÀÎ Sensi-view Ubimax reagent »ç¿ë: ¹Ì·®´Ü¹éÁú °ËÃâÇÑ°è ±Øº¹, non-specific band ÃÖ¼ÒÈ
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Service Type | Content | ±âÁØ | Price (¿ø) |
---|---|---|---|
Sample Prep | Cell pellet lysis IP prep (Secreted protein) Tissue homogenation |
One sample | 5,000 25,000 15,000 |
Protein Quantification | BCA quantification | One sample | 7,000 |
WB set-up charge | 7 ~ 8 samples | One blot | 350,000 |
Gel staining | Coomasie/Silver staining | One blot | 40,000 |
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- Cytosol & Nuclear Protein: > 3 x 10^6 Frozen cells / Sample
- Secreted Protein: Serum-free Media, Albumin-removed Media Sample
- * »ùÇüö°Å : µ¿°á»óÅ »ùÇà ȸ¼ö ¿øÄ¢
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Cat No. LGW-1001-200, Sensi view¢â Pico ECL Western Reagent Cat No. LGW-1001-500, Sensi view¢â Pico ECL Western Reagent Cat No. LGW-P1001-200, Sensi view¢â Pico Plus ECL Western Reagent Cat No. LGW-P1001-500, Sensi view¢â Pico Plus ECL Western Reagent Cat No. LGW-1002-100, Sensi-view¢â Ubimax reagent Cat No. LGW-1002-500, Sensi-view¢â Ubimax reagent Cat No. LGM-1102, Lugen Sensi color¢â Protein Marker (Bio-rad Type) Cat No. LGM-1102B, Lugen Sensi color¢â Protein Marker (Bio-rad Type)