Product name: SOCS1-KIR

Sequence: DTHFRTFRSHSDYRRI

Purity: 95% by HPLC

Counter ion: Trifluoroacetate

Format: Lyophilized powder

Description: Tkip and a peptide corresponding to the KIR of Suppressor of cytokine signaling (SOCS)-1 protein, ((53))DTHFRTFRSHSDYRRI((68)) (SOCS1-KIR), both bound similarly to the autophosphorylation site of JAK2, JAK2(1001-1013). The peptides also bound to JAK2 peptide phosphorylated at Tyr(1007), pJAK2(1001-1013). Dose-response competitions suggest that Tkip and SOCS1-KIR similarly recognize the autophosphorylation site of JAK2, but probably not precisely the same way. Although Tkip inhibited JAK2 autophosphorylation as well as IFN-gamma-induced STAT1-alpha phosphorylation, SOCS1-KIR, like SOCS-1, did not inhibit JAK2 autophosphorylation but inhibited STAT1-alpha activation. Both Tkip and SOCS1-KIR inhibited IFN-gamma activation of Raw 264.7 murine macrophages and inhibited Ag-specific splenocyte proliferation. The fact that SOCS1-KIR binds to pJAK2(1001-1013) suggests that the JAK2 peptide could function as an antagonist of SOCS-1.

Usage: For Scientific Research Use Only, Not for Human Use.

Reference:

J Immunol. 2007 Apr 15;178(8):5058-68.