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카테고리
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Chemokines
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CAT.NO
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LGP-20-027
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PRODUCT
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MCP-2/CCL8, Human (Monocyte Chemotactic Protein-2/CCL8)
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SIZE
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10ug, 100ug, 500ug
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PRICE
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KRW 285,000, 880,000, 2,569,000
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Technical Parameters
| Synonyms |
HC14, Small-inducible cytokine A8 |
| Species |
9 |
| Accession |
P80075 |
| GeneID |
6355 |
| Source |
Escherichia coli. |
| Molecular Weight |
Approximately 8.9 kDa, a single non-glycosylated polypeptide chain containing 76 amino acids. |
| Quantity |
10µg/100µg/500µg |
| AA Sequence |
QPDSVSIPIT CCFNVINRKI PIQRLESYTR ITNIQCPKEA VIFKTKRGKE VCADPKERWV RDSMKHLDQI FQNLKP |
| Purity |
> 96 % by SDS-PAGE and HPLC analyses. |
| Biological Activity |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-100 ng/ml. |
| Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl. |
| Endotoxin |
Less than 1 EU/μg of rHuMCP-2/CCL8 as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Usage |
This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
| SDS-PAGE |
 |
| Reference |
1. Van Coillie E, Fiten P, Nomiyama H, et al. 1997. Genomics. 40:323-31.
2. Opdenakker G, Froyen G, Fiten P, et al. 1993. Biochem Biophys Res Commun. 191:535-42.
3. Proost P, Wuyts A, Van Damme J. 1996. J Leukoc Biol. 59:67-74.
4. Gong W, Howard OM, Turpin JA, et al. 1998. J Biol Chem. 273:4289-92. |
| Background |
Human CCL8, also known as monocyte chemotactic protein 2 (MCP-2), is belonging to the CC chemokine family. It is encoded by the gene CCL8. MCP-2 has two homogeneous MCP-1 (CCL2) and MCP-3 (CCL7). These three MCPs were found by IL-1-beta triggered human MG-63 osteosarcoma cells. CCL8 shares 62 % amino acid sequence identity with MCP-1, and shares 58 % amino acid sequence identity with MCP-2. CCL8 has chemotactic function for monocytes, eosinophils and neutrophils. In addition, it can also chemoattract activated NK cells. |
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