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카테고리 Enzymes & Inhibitors
CAT.NO LGP-40-007
PRODUCT TIGAR-TAT, Human (TP53-induced Glycolysis and Apoptosis Regulator-TAT)
SIZE 25ug, 100ug, 500ug
PRICE KRW 285,000, 880,000, 2,569,000
Technical Parameters
Species 9
Accession Q9NQ88
GeneID 57103
Source Escherichia coli.
Molecular Weight Approximately 31.7 kDa, a single non-glycosylated polypeptide chain containing 283 amino acids.
Quantity 25µg/100µg/500µg
AA Sequence MARFALTVVR HGETRFNKEK IIQGQGVDEP LSETGFKQAA AAGIFLNNVK FTHAFSSDLM RTKQTMHGIL ERSKFCKDMT VKYDSRLRER KYGVVEGKAL SELRAMAKAA REECPVFTPP GGETLDQVKM RGIDFFEFLC QLILKEADQK EQFSQGSPSN CLETSLAEIF PLGKNHSSKV NSDSGIPGLA ASVLVVSHGA YMRSLFDYFL TDLKCSLPAT LSRSELMSVT PNTGMSLFII NFEEGREVKP TVQCICMNLQ DHLNGLTETR GGYGRKKRRQ RRR
Purity > 96 % by SDS-PAGE and HPLC analyses.
Biological Activity Fully biologically active when compared to standard. The biological activity determined by its ability to protect U2OS cells from apoptosis induced by hydrogen peroxide is in a concentration range of 0.1-5.0 μg/ml, after pretreating with rHuTIGAR-TAT for 4 hours.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 30 % Acetonitrile, 0.1% TFA.
Endotoxin Less than 0.1 EU/μg of rHuTIGAR-TAT as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Usage This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
SDS-PAGE
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