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카테고리
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Others
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CAT.NO
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LGP-62-008
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PRODUCT
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Murine Fatty-acid-binding Protein 1 (FABP1, Mouse)
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SIZE
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25ug, 100ug, 500ug
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PRICE
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KRW 285,000, 870,000, 2,560,000
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Technical Parameters
| Synonyms |
14 kDa Selenium-binding Protein, Fatty Acid-binding Protein 1, L-FABP |
| Accession |
P12710 |
| GeneID |
14080 |
| Source |
Escherichia coli. |
| Molecular Weight |
Approximately 14.2 kDa, a single non-glycosylated polypeptide chain containing 127 amino acids. |
| Quantity |
25µg/100µg/500µg |
| AA Sequence |
MNFSGKYQLQ SQENFEPFMK AIGLPEDLIQ KGKDIKGVSE IVHEGKKIKL TITYGPKVVR NEFTLGEECE LETMTGEKVK AVVKLEGDNK MVTTFKGIKS VTELNGDTIT NTMTLGDIVY KRVSKRI |
| Purity |
> 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity |
Fully biologically active when compared to standard. The binding affinity of rMuFABP1 for the synthetic ligand cis-parinaric acid has been measured by fluorescence titration. Half maximal fluorescence of 2.5 μM rMuFABP1 is achieved with approximately 5 μM cis-paranaric acid. |
| Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4, 2 % trehalose. |
| Endotoxin |
Less than 1 EU/µg of rMuFABP1 as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Usage |
This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
| SDS-PAGE |
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| Reference |
1. Chmurzynska A. 2006. J Appl Genet. 47:39-48.
2. Weisiger RA. 2002. Mol Cell Biochem. 239:35-43.
3. Chen SH, Van Tuinen P, Ledbetter DH, et al. 1986. Somat Cell Mol Genet. 12:303-6.
4. Weickert MO, Loeffelholz CV, Roden M, et al. 2007. Am J Physiol Endocrinol Metab. 293:E1078-84.
5. Bassuk JA, Tsichlis PN, Sorof S. 1987. Proc Natl Acad Sci U S A. 84:7547-51.
6. He Y, Yang X, Wang H, et al. 2007. Biochemistry. 46:12543-56. |
| Background |
The fatty-acid-binding proteins (FABPs) are a family of carrier proteins for fatty acids and other lipophilic substances such as eicosanoids and retinoids. These proteins are thought to facilitate the transfer of fatty acids between extra- and intracellular membranes. Fatty acid-binding protein 1 (FABP1) encoded by the FABP1 gene, also known as liver-type fatty acid-binding protein (L-FABP), is a member of FABP family and it is a small, highly conserved, cytoplasmic proteins. In addition, FABP1 binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. Furthermore, it may be involved in intracellular lipid transport. Through amino acid sequence comparison, murine FABP1 shares 84 % and 94 % a.a. sequence identity with human and rat FABP1. |
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