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카테고리
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Others
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CAT.NO
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LGP-64-003
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PRODUCT
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Migration Inhibitor Factor (MIF, Rat)
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SIZE
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50ug, 250ug,1mg
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PRICE
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KRW 285,000, 1,000,000, 3,300,000
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Technical Parameters
| Synonyms |
MIF, Glutathione-binding 13 kDa Protein, L-dopachrome Isomerase, L-dopachrome Tautomerase, Phenylpyruvate Tautomerase |
| Accession |
P30904 |
| GeneID |
81683 |
| Source |
Escherichia coli. |
| Molecular Weight |
Approximately 12.5 kDa, a single non-glycosylated polypeptide chain containing 115 amino acids. |
| Quantity |
50µg/250µg/1000µg |
| AA Sequence |
MPMFIVNTNV PRASVPEGFL SELTQQLAQA TGKPAQYIAV HVVPDQLMTF SGTSDPCALC SLHSIGKIGG AQNRNYSKLL CGLLSDRLHI SPDRVYINYY DMNAANVGWN GSTFA |
| Purity |
> 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity |
Test in process. |
| Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM Tris, pH 8.0, 150 mM NaCl, 3 % trehalose. |
| Endotoxin |
Less than 1 EU/µg of rRtMIF as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Usage |
This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
| SDS-PAGE |
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| Reference |
1. WY Weiser, PA Temple, JS Witek-Giannotti, et al. 1989. Proc Natl Acad Sci U S A, 86: 7522-6.
2. CA Kozak, MC Adamson, CE Buckler, et al. 1995. Genomics, 27: 405-11.
3. DF LarsonandK Horak. 2006. Crit Care, 10: 138.
4. HW Sun, J Bernhagen, R Bucala, et al. 1996. Proc Natl Acad Sci U S A, 93: 5191-6.
5. M Oddo, T Calandra, R Bucala, et al. 2005. Infect Immun, 73: 3783-6.
6. M Emonts, FC Sweep, N Grebenchtchikov, et al. 2007. Clin Infect Dis, 44: 1321-8. |
| Background |
Macrophage migration inhibitory factor (MIF or MMIF), also named as glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, or phenylpyruvate tautomerase, is a protein encoded by the MIF gene. It is released from white blood cells by bacterial antigen stimulation to trigger an acute immune response, or by glucocorticoids to counter-act the inhibitory effects of glucocorticoids on immune system. MIF is a homotrimer of which each subunit contains 115 amino acids. As mentioned above, MIF is involved in the innate immune response to bacterial pathogens and counter-acts the anti-inflammatory activity of glucocorticoids. Furthermore, it also plays a role as mediator in regulating the function of macrophages in host defense and has phenylpyruvate tautomerase and dopachrome tautomerase activity in vitro. Rat MIF is 99 %, 90 %, 89 %, and 89 % a.a. identical to human, murine, porcine and bovine, respectively. |
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