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카테고리
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Others
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CAT.NO
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LGP-65-001
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PRODUCT
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Recombinant Exendin-4
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SIZE
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100ug, 500ug, 1mg
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PRICE
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KRW 285,000, 470,000, 642,000
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Technical Parameters
| Synonyms |
Exenatide |
| Accession |
P26349 |
| Source |
Escherichia coli. |
| Molecular Weight |
Approximately 4.2 kDa, a single non-glycosylated polypeptide chain containing 39 amino acids. |
| Quantity |
100µg/500µg/1000µg |
| AA Sequence |
HGEGTFTSDL SKQMEEEAVR LFIEWLKNGG PSSGAPPPS |
| Purity |
> 96 % by SDS-PAGE and HPLC analyses. |
| Biological Activity |
1. Regulates Glucose levels rapidly;
2. Reduces Insulin resistance;
3. Reduces Glucagon;
4. Reduces HbA1c;
5. Stimulates beta cell growth which stimulates insulin production. |
| Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 µm filtered solution in PBS, pH 7.4. |
| Endotoxin |
Less than 10 EU/mg of rExendin-4 as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Usage |
This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
| SDS-PAGE |
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| Reference |
1. Malhotra R, Singh L, Eng J, et al. 1992. Regul Pept, 41: 149-56.
2. Neidigh JW, Fesinmeyer RM, Prickett KS, et al. 2001. Biochemistry, 40: 13188-200.
3. Movassat J, Beattie GM, Lopez AD, et al. 2002. J Clin Endocrinol Metab, 87: 4775-81.
4. Alarcon C, Wicksteed B, Rhodes CJ. 2006. Diabetologia, 49: 2920-9. |
| Background |
Exendin-4 is a novel 39-amino acid peptide isolated from the venom of the Gila monster Heloderma suspectum. It shares 53% sequence homology with GLP-17-36amide and interacts with the same membrane receptor. Exendin-4 enhances glucose-dependent insulin secretion, suppresses inappropriately elevated glucagon secretion, and slows gastric emptying in vivo. It also promotes ß-cell proliferation and neogenesis in vitro and in animal models. Recombinant Exendin-4 is E. coli expression of a synthetic DNA squence encoding the 39 amino acid of Exendin-4. |
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