Ä«Å×°í¸®
|
Cytokines & Growth Factor
|
CAT.NO
|
LGP-11-008
|
PRODUCT
|
IL-16, Rhesus (Macaque Interleukin-16)
|
SIZE
|
10ug, 100ug, 250ug
|
PRICE
|
KRW 285,000, 1,522,000, 2,622,000
|
Technical Parameters
Synonyms |
Interleukin-16, Lymphocyte Chemoattractant Factor, LCF |
Accession |
O62675 |
GeneID |
574100 |
Source |
Escherichia coli. |
Molecular Weight |
Approximately 12.5 kDa, a single non-glycosylated polypeptide chain containing 121 amino acids. |
Quantity |
10µg/100µg/250µg |
AA Sequence |
SAASASAASD VSVESSAEAT VYTVTLEKMS AGLGFSLEGG KGSLHGDKPL TINRIFKGAA SEQSETIQPG DEILQLAGTA MQGLTRFEAW NIIKALPDGP VTIVIRRKSL QPKETTAAAD S |
Purity |
> 98 % by SDS-PAGE and HPLC analyses. |
Biological Activity |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral T lymphocytes is in a concentration range of 1.0-100 ng/ml. |
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4. |
Endotoxin |
Less than 1 EU/µg of rRhIL-16 as determined by LAL method. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ¡Â -20 ¡ÆC. Further dilutions should be made in appropriate buffered solutions. |
Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 ¡ÆC as supplied. - 1 month, 2 to 8 ¡ÆC under sterile conditions after reconstitution. - 3 months, -20 to -70 ¡ÆC under sterile conditions after reconstitution. |
Usage |
This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
SDS-PAGE |
|
Reference |
1. Baier M, Bannert N, Werner A, et al. 1997. Proc Natl Acad Sci U S A, 94: 5273-7. 2. Zhang Y, Center DM, Wu DM, et al. 1998. J Biol Chem, 273: 1144-9. 3. Cruikshank WW, Kornfeld H, Center DM. 2000. J Leukoc Biol, 67: 757-66. 4. Cruikshank WW, Center DM, Nisar N, et al. 1994. Proc Natl Acad Sci U S A, 91: 5109-13. 5. Mathy NL, Scheuer W, Lanzendorfer M, et al. 2000. Immunology, 100: 63-9. |
Background |
Interleukin-16 (IL-16) is also called lymphocyte chemoattractant factor (LCF) and it is mostly secreted by lymphocytes, epithelial cells, eosinophils, and CD8+ T cells. It has many functions including induction of the IL-2R¥á on T cells, suppression of human immunodeficiency virus (HIV) replication, inhibition of T-cell antigen receptor/CD3 mediated T-cell stimulation in mixed lymphocyte reactions and so on. It signals through CD4 receptor. Furthermore, recombinant rhesus macaque interleukin-16 contains 121 amino acid residues and it shares 85 % ~ 95 % a.a. sequence identity with human and murine IL-16. |
|
÷ºÎÆÄÀÏ
|
|
|
|
|