Ä«Å×°í¸®
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Cytokines & Growth Factor
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CAT.NO
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LGP-12-003
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PRODUCT
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IL-1RA, Mouse (Murine Interleukin-1 Receptor Antagonist Protein)
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SIZE
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100ug, 500ug, 1mg
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PRICE
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KRW 285,000, 940,000, 1,570,000
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Technical Parameters
Synonyms |
IL-1RN, IRAP |
Accession |
Q542W1 |
GeneID |
16181 |
Source |
Escherichia coli. |
Molecular Weight |
Approximately 17.3 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids. |
Quantity |
100µg/500µg/1000µg |
AA Sequence |
RPSGKRPCKM QAFRIWDTNQ KTFYLRNNQL IAGYLQGPNI KLEEKIDMVP IDLHSVFLGI HGGKLCLSCA KSGDDIKLQL EEVNITDLSK NKEEDKRFTF IRSEKGPTTS FESAACPGWF LCTTLEADRP VSLTNTPEEP LIVTKFYFQE DQ |
Purity |
> 97 % by SDS-PAGE and HPLC analyses. |
Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-1¥á-dependent proliferation of murine D10S cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 ¡¿ 104 IU/mg in the presence of 50 pg/ml rHuIL-1¥á. |
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4. |
Endotoxin |
Less than 1 EU/µg of rMuIL-1RA as determined by LAL method. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ¡Â -20 ¡ÆC. Further dilutions should be made in appropriate buffered solutions. |
Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 ¡ÆC as supplied. - 1 month, 2 to 8 ¡ÆC under sterile conditions after reconstitution. - 3 months, -20 to -70 ¡ÆC under sterile conditions after reconstitution. |
Usage |
This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
SDS-PAGE |
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Reference |
1. Dinarello CA. 1994. FASEB J. 8:1314-25. 2. Patterson D, Jones C, Hart I, et al. 1993. Genomics. 15:173-6. 3. Steinkasserer A, Spurr NK, Cox S, et al. 1992. Genomics. 13:654-7. 4. Perrier S, Darakhshan F, Hajduch E. 2006. FEBS Lett. 580:6289-94. 5. Gabay C, Porter B, Fantuzzi G, et al. 1997. J Immunol. 159:5905-13. |
Background |
IL-1RA was initially called the IL-1 inhibitor which is encoded by the IL1RN gene and it is a member of the interleukin 1 cytokine family. IL-1RA is secreted by various types of cells including immune cells, epithelial cells, and adipocytes. IL-RA has functions of inhibiting the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. IL-1RA is also used in the treatment of rheumatoid arthritis, an autoimmune disease in which IL-1 plays a key role. The murine IL-1RA is a single non-glycosylated polypeptide chain containing 152 amino acids and it has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. The protein shows 26 % amino acid homology to IL-1¥â and 19% homology to IL-1¥á. It also shares 90 % a.a. sequence identity with rat IL-1RA. |
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