Ä«Å×°í¸®
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Cytokines & Growth Factor
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CAT.NO
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LGP-12-005
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PRODUCT
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IL-3, Mouse (Murine Interleukin-3)
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SIZE
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10ug, 100ug, 500ug
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PRICE
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KRW 285,000, 880,000, 2,569,000
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Technical Parameters
Synonyms |
Hematopoietic growth factor, MCGF, Multipotential colony-stimulating factor, P-cell-stimulating factor. |
Accession |
P01586 |
GeneID |
16187 |
Source |
Escherichia coli. |
Molecular Weight |
Approximately 14.8 kDa globular protein containing 134 amino acid residues. |
Quantity |
10µg/100µg/500µg |
AA Sequence |
DTHRLTRTLN CSSIVKEIIG KLPEPELKTD DEGPSLRNKS FRRVNLSKFV ESQGEVDPED RYVIKSNLQK LNCCLPTSAN DSALPGVFIR DLDDFRKKLR FYMVHLNDLE TVLTSRPPQP ASGSVSPNRG TVEC |
Purity |
> 98 % by SDS-PAGE and HPLC analyses. |
Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2 ¡¿ 107 IU/mg. |
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation |
Lyophilized from a 0.2 µm filtered solution in PBS, pH 7.4. |
Endotoxin |
Less than 1 EU/µg of rMuIL-3 as determined by LAL method. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ¡Â -20 ¡ÆC. Further dilutions should be made in appropriate buffered solutions. |
Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 ¡ÆC as supplied. - 1 month, 2 to 8 ¡ÆC under sterile conditions after reconstitution. - 3 months, -20 to -70 ¡ÆC under sterile conditions after reconstitution. |
Usage |
This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
SDS-PAGE |
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Reference |
1. Yang YC, Ciarletta AB, Temple PA, et al. 1986. Cell. 47:3-10. 2. Otsuka T, Miyajima A, Brown N, et al. 1988. J Immunol. 140:2288-95. 3. Miyatake S, Yokota T, Lee F, et al. 1985. Proc Natl Acad Sci U S A. 82:316-20. 4. Dorssers L, Burger H, Bot F, et al. 1987. Gene. 55:115-24. |
Background |
Interleukin-3 (IL-3) is a type of biological signal (cytokine) which is encoded by the IL-3 gene located on chromosome 5 and produced primarily by activated T cells beside human thymic epithelial cells, activated murine mast cells, murine keratinocytes and neurons/astrocytes. The protein acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. In addition, it exerts its biological activities through binding to interleukin-3 receptors included ¥á and ¥â subunits. The Mouse IL-3 is different from human IL-3 and contains 140 amino acids residues. Specifically, mouse and human IL-3 share low homology and have not cross species activity. |
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