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카테고리
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Cytokines & Growth Factor
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CAT.NO
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LGP-12-011
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PRODUCT
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IL-10, Mouse (Murine Interleukin-10)
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SIZE
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10ug, 100ug, 250ug
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PRICE
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KRW 285,000, 1,522,000, 2,622,000
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Technical Parameters
| Synonyms |
CSIF |
| Accession |
P18893 |
| GeneID |
16153 |
| Source |
Escherichia coli. |
| Molecular Weight |
Approximately 18.7 kDa, a single non-glycosylated polypeptide chain containing 160 amino acids. |
| Quantity |
10µg/100µg/250µg |
| AA Sequence |
SRGQYSREDN NCTHFPVGQS HMLLELRTAF SQVKTFFQTK DQLDNILLTD SLMQDFKGYL GCQALSEMIQ FYLVEVMPQA EKHGPEIKEH LNSLGEKLKT LRMRLRRCHR FLPCENKSKA VEQVKSDFNK LQDQGVYKAM NEFDIFINCI EAYMMIKMKS |
| Purity |
> 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine MC/9-2 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0 × 106 IU/mg. |
| Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 µm filtered solution in PBS, pH 7.4. |
| Endotoxin |
Less than 1 EU/µg of rMuIL-10 as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10 mM HCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Usage |
This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
| SDS-PAGE |
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| Reference |
1. Kim JM, Brannan CI, Copeland NG, et al. 1992. J Immunol. 148:3618-23.
2. Tan JC, Braun S, Rong H, et al. 1995. J Biol Chem. 270:12906-11.
3. Pestka S, Krause CD, Sarkar D, et al. 2004. Annu Rev Immunol. 22:929-79.
4. Vieira P, de Waal-Malefyt R, Dang MN, et al. 1991. Proc Natl Acad Sci U S A. 88:1172-6.
5. Hsu DH, de Waal Malefyt R, Fiorentino DF, et al. 1990. Science. 250:830-2. |
| Background |
Interleukin-10 (IL-10) is encoded by the IL10 gene and expressed in monocytes and Type 2 T helper cells (Th2). The biological activities of IL-10 are mediated by the IL-10 receptor complex, which is composed of the ligand-binding IL-10 Rα/R1 and the accessory IL-10 Rβ/R2 subunits. IL-10 is capable of inhibiting the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by T helper cells. IL-10 is classified as a class 2 cytokine which consist of IL-10, IL-19, IL-20, IL-22, IL-24 and IL-26, several interferons and interferon-like molecules. Mature murine IL-10 shares 70 %-77 % amino acid sequence identity with human, bovine, porcine IL-10, but murine IL-10 does not act on human cells. |
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