Ä«Å×°í¸®
|
Cytokines & Growth Factor
|
CAT.NO
|
LGP-12-012
|
PRODUCT
|
IL-11, Mouse (Murine Interleukin-11)
|
SIZE
|
10ug, 100ug, 250ug
|
PRICE
|
KRW 285,000, 1,522,000, 2,622,000
|
Technical Parameters
Accession |
P47873 |
GeneID |
16156 |
Source |
Escherichia coli. |
Molecular Weight |
Approximately 19.1 kDa, a single non-glycosylated polypeptide chain containing 179 amino acids. |
Quantity |
10µg/100µg/250µg |
AA Sequence |
MPGPPAGSPR VSSDPRADLD SAVLLTRSLL ADTRQLAAQM RDKFPADGDH SLDSLPTLAM SAGTLGSLQL PGVLTRLRVD LMSYLRHVQW LRRAGGPSLK TLEPELGALQ ARLERLLRRL QLLMSRLALP QAAPDQPVIP LGPPASAWGS IRAAHAILGG LHLTLDWAVR GLLLLKTRL |
Purity |
> 97 % by SDS-PAGE and HPLC analyses. |
Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine T11 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 ¡¿ 105 IU/mg. |
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation |
Lyophilized from a 0.2 µm filtered solution in PBS, pH 7.4. |
Endotoxin |
Less than 1 EU/µg of rMuIL-11 as determined by LAL method. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ¡Â -20 ¡ÆC. Further dilutions should be made in appropriate buffered solutions. |
Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 ¡ÆC as supplied. - 1 month, 2 to 8 ¡ÆC under sterile conditions after reconstitution. - 3 months, -20 to -70 ¡ÆC under sterile conditions after reconstitution. |
Usage |
This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
SDS-PAGE |
|
Reference |
1. Paul SR, Bennett F, Calvetti JA, et al. 1990. Proc Natl Acad Sci U S A. 87:7512-6. 2. Barton VA, Hall MA, Hudson KR, et al. 2000. J Biol Chem. 275:36197-203. |
Background |
Interleukin-11 (IL-11) is encoded by the II11 gene. IL-11 is a multifunctional cytokine first isolated from bone marrow-derived stromal cells. IL-11 receptor activation requires formation of a complex of two IL-11 molecules with two molecules of the ligand-binding IL-11 R¥á subunit and two molecules of the expressed cell signaling ¥â subunit, gp130. IL-11 is a member of the IL-6 superfamily, distinguished based on their use of the common co-receptor gp130. IL-11 can directly stimulate the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells, and induce megakaryocyte maturation resulting in increased platelet production. Mature murine IL-11 shares 88 % amino acid sequence identity with human IL-11. |
|
÷ºÎÆÄÀÏ
|
|
|
|
|