Ä«Å×°í¸®
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Cytokines & Growth Factor
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CAT.NO
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LGP-12-014
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PRODUCT
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IL-16(121a.a.), Mouse (Murine Interleukin-16, 121aa)
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SIZE
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10ug, 100ug, 250ug
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PRICE
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KRW 285,000, 1,522,000, 2,622,000
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Technical Parameters
Synonyms |
Lymphocyte chemoattractant factor , LCF |
Accession |
O54824 |
GeneID |
16170 |
Source |
Escherichia coli. |
Molecular Weight |
Approximately 12.2 kDa, a single non-glycosylated polypeptide chain containing 118 amino acids. |
Quantity |
10µg/100µg/250µg |
AA Sequence |
SAASASAASD ISVESKEATV CTVTLEKTSA GLGFSLEGGK GSLHGDKPLT INRIFKGTEQ GEMVQPGDEI LQLAGTAVQG LTRFEAWNVI KALPDGPVTI VIRRTSLQCK QTTASADS |
Purity |
> 98 % by SDS-PAGE and HPLC analyses. |
Biological Activity |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral T lymphocytes is in a concentration range of 1.0-100 ng/ml. |
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4. |
Endotoxin |
Less than 1 EU/µg of rMuIL-16 as determined by LAL method. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ¡Â -20 ¡ÆC. Further dilutions should be made in appropriate buffered solutions. |
Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 ¡ÆC as supplied. - 1 month, 2 to 8 ¡ÆC under sterile conditions after reconstitution. - 3 months, -20 to -70 ¡ÆC under sterile conditions after reconstitution. |
Usage |
This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
SDS-PAGE |
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Reference |
1. Baier M, Bannert N, Werner A, et al. 1997. Proc Natl Acad Sci U S A, 94: 5273-7. 2. Zhang Y, Center DM, Wu DM, et al. 1998. J Biol Chem, 273: 1144-9. 3. Cruikshank WW, Kornfeld H, Center DM. 2000. J Leukoc Biol, 67: 757-66. 4. Cruikshank WW, Center DM, Nisar N, et al. 1994. Proc Natl Acad Sci U S A, 91: 5109-13. 5. Mathy NL, Scheuer W, Lanzendorfer M, et al. 2000. Immunology, 100: 63-9. |
Background |
Interleukin-16 (IL-16) is also called lymphocyte chemoattractant factor (LCF) and it is mostly secreted by lymphocytes, epithelial cells, eosinophils, and CD8+ T cells. It has many functions including induction of the IL-2R¥á on T cells, suppression of human immunodeficiency virus (HIV) replication, inhibition of T-cell antigen receptor/CD3 mediated T-cell stimulation in mixed lymphocyte reactions and so on. It signals through CD4 receptor. Furthermore, recombinant murine interleukin-16 contains 118 amino acid residues and it shares about 85 % a.a. sequence identity with different isoforms of human IL-16. |
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