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카테고리
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Cytokines & Growth Factor
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CAT.NO
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LGP-12-024
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PRODUCT
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IL-36RA, Mouse (Murine Interleukin-36 Receptor Antagonist Protein)
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SIZE
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10ug, 100ug, 500ug
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PRICE
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KRW 285,000, 880,000, 2,569,000
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Technical Parameters
| Synonyms |
IL-1HY1, IL-1 delta, IL-1F5, IL-1H3, IL-1L1 |
| Accession |
Q9QYY1 |
| GeneID |
54450 |
| Source |
Escherichia coli. |
| Molecular Weight |
Approximately 16.9 kDa, a single non-glycosylated polypeptide chain containing 154 amino acids. |
| Quantity |
10µg/100µg/500µg |
| AA Sequence |
VLSGALCFRM KDSALKVLYL HNNQLLAGGL HAEKVIKGEE ISVVPNRALD ASLSPVILGV QGGSQCLSCG TEKGPILKLE PVNIMELYLG AKESKSFTFY RRDMGLTSSF ESAAYPGWFL CTSPEADQPV RLTQIPEDPA WDAPITDFYF QQCD |
| Purity |
> 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity |
Measured by its ability to inhibit IL-36γ induced IL-6 production by human PBMCs. |
| Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin |
Less than 1 EU/µg of rMuIL-36RA as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Usage |
This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
| Reference |
1. Born TL, Smith DE, Garka KE, et al. 2000. J Biol Chem. 275:41528.
2. Dunn EF, Gay NJ, Bristow AF, et al. 2003. Biochemistry. 42:10938-44.
3. Barton JL, Herbst R, Bosisio D, et al. 2000. Eur J Immunol. 30:3299-308.
4. Debets R, Timans JC, Homey B, et al. 2001. J Immunol. 167:1440-6.
5. Towne JE, Renshaw BR, Douangpanya J, et al. 2011. J Biol Chem. 286:42594-602. |
| Background |
Interleukin-36 receptor antagonist (IL-36RA) is a secreted protein which belongs to the interleukin 1 cytokine family (IL-1 family) and it is predominantly expressed in keratinocytes but not in fibroblasts, endothelial cells or melanocytes. IL-36RA has been reported to antagonize the biological activity of IL-36α (IL-1F6), IL-36β (IL-1F8), and IL-36γ (IL-1F9). Furthermore, it could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL-1R1). In addition, The receptor for IL-36RA has not been positively identified. Indirect evidence suggests it is IL-1Rrp2. Recombinant murine interleukin-36 RA contains 154 amino acid residues and it shares 91 % a.a. sequence identity with human IL-36RA. |
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