Ä«Å×°í¸®
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Cytokines & Growth Factor
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CAT.NO
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LGP-12-034
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PRODUCT
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TPO, Mouse (Murine Thrombopoietin)
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SIZE
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10ug, 100ug, 250ug
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PRICE
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KRW 285,000, 1,247,000, 2,170,000
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Technical Parameters
Accession |
P40226 |
GeneID |
21832 |
Source |
Escherichia coli. |
Molecular Weight |
Approximately 18.7 kDa, a single non-glycosylated polypeptide chain containing 174 amino acids. |
Quantity |
10µg/100µg/250µg |
AA Sequence |
SPVAPACDPR LLNKLLRDSH LLHSRLSQCP DVDPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPLQGRTTA HKDPNALFLS LQQLLRGKVR FLLLVEGPTL CVRRTLPTTA VPSSTSQLLT LNKF |
Purity |
> 95 % by SDS-PAGE and HPLC analyses. |
Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human MO7e cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 ¡¿ 106 IU/mg. |
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4, with 5% Trehalose. |
Endotoxin |
Less than 0.1 EU/µg of rMuTPO as determined by LAL method. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ¡Â -20 ¡ÆC. Further dilutions should be made in appropriate buffered solutions. |
Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 ¡ÆC as supplied. - 1 month, 2 to 8 ¡ÆC under sterile conditions after reconstitution. - 3 months, -20 to -70 ¡ÆC under sterile conditions after reconstitution. |
Usage |
This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
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