Ä«Å×°í¸®
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Cytokines & Growth Factor
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CAT.NO
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LGP-12-036
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PRODUCT
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TNF-¥á/TNFSF2, Mouse (Murine Tumor Necrosis Factor-alpha/TNFSF2)
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SIZE
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20ug, 100ug, 500ug
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PRICE
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KRW 285,000, 880,000, 2,569,000
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Technical Parameters
Synonyms |
Tumor Necrosis Factor, TNFSF2, Cachectin, Differentiation-inducing factor , DIF, Necrosin, Cytotoxin |
Accession |
P06804 |
GeneID |
21926 |
Source |
Escherichia coli. |
Molecular Weight |
Approximately 17.4 kDa. The recombinant murine TNF-¥á is a soluble 157 amino acid protein which corresponds to C-terminal extracellular domain of the full length transmembrane protein. |
Quantity |
20µg/100µg/500µg |
AA Sequence |
MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL |
Purity |
> 98 % by SDS-PAGE and HPLC analyses. |
Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 ¡¿ 107 IU/mg in the presence of actinomycin D. |
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation |
Lyophilized from a 0.2 ¥ìm filtered solution in PBS, pH 7.2. |
Endotoxin |
Less than 1 EU/¥ìg of rMuTNF-¥á as determined by LAL method. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ¡Â -20 ¡ÆC. Further dilutions should be made in appropriate buffered solutions. |
Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 ¡ÆC as supplied. - 1 month, 2 to 8 ¡ÆC under sterile conditions after reconstitution. - 3 months, -20 to -70 ¡ÆC under sterile conditions after reconstitution. |
Usage |
This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
SDS-PAGE |
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Reference |
1. Davenport C, Kenny H, Ashley DT, et al. 2012. Eur J Clin Invest, 42: 1173-9. 2. Cavalcanti YV, Brelaz MC, Neves JK, et al. 2012. Pulm Med, 2012: 745483. 3. Sheng WS, Hu S, Ni HT, et al. 2005. J Leukoc Biol, 78: 1233-41. 4. Berthold-Losleben MandHimmerich H. 2008. Curr Neuropharmacol, 6: 193-202. |
Background |
Tumor necrosis factor alpha (TNF-¥á), also called cachectin, is the best-know member of the TNF-family, which can cause cell death. This protein is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-¥á occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF-¥á is glycosylated, but non-glycosylated recombinant TNF-¥á has comparable biological activity. The biologically active native form of TNF-¥á is reportedly a trimer. Human and murine TNF-¥á show approximately 79 % homology at the amino acid level and cross-reactivity between the two species. Two types of receptors for TNF-¥á have been described and virtually all cell types studied show the presence of one or both of these receptor types. |
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