Ä«Å×°í¸®
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Cytokines & Growth Factor
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CAT.NO
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LGP-12-054
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PRODUCT
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KGF-1/FGF-7, Mouse (Murine Keratinocyte Growth Factor 1/FGF-7)
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SIZE
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10ug, 100ug, 250ug
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PRICE
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KRW 285,000, 1,522,000, 2,622,000
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Technical Parameters
Synonyms |
,HBGF-7 |
Accession |
P36363 |
GeneID |
14178 |
Source |
Escherichia coli. |
Molecular Weight |
Approximately 18.7 kDa, a single, non-glycosylated polypeptide chain containing 163 amino acids. |
Quantity |
10µg/100µg/250µg |
AA Sequence |
CNDMSPEQTA TSVNCSSPER HTRSYDYMEG GDIRVRRLFC RTQWYLRIDK RGKVKGTQEM KNSYNIMEIR TVAVGIVAIK GVESEYYLAM NKEGKLYAKK ECNEDCNFKE LILENHYNTY ASAKWTHSGG EMFVALNQKG IPVKGKKTKK EQKTAHFLPM AIT |
Purity |
> 96 % by SDS-PAGE and HPLC analyses. |
Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0 ¡¿ 105 IU/mg. |
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation |
Lyophilized from a 0.2 µm filtered solution in 20 mM PB, pH 8.0, 1 M NaCl. |
Endotoxin |
Less than 1 EU/µg of rMuKGF-1/FGF-7 as determined by LAL method. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ¡Â -20 ¡ÆC. Further dilutions should be made in appropriate buffered solutions. |
Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 ¡ÆC as supplied. - 1 month, 2 to 8 ¡ÆC under sterile conditions after reconstitution. - 3 months, -20 to -70 ¡ÆC under sterile conditions after reconstitution. |
Usage |
This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
Reference |
1. Mattei MG, deLapeyriere O, Bresnick J, et al. 1995. Mamm Genome. 6:196-7. 2. Kelley MJ, Pech M, Seuanez HN, et al. 1992. Proc Natl Acad Sci U S A. 89:9287-91. 3. de Giorgi V, Sestini S, Massi D, et al. 2007. Dermatol Clin. 25:477-85, vii. 4. Eswarakumar VP, Lax I, Schlessinger J. 2005. Cytokine Growth Factor Rev. 16:139-49. 5. Belleudi F, Leone L, Nobili V, et al. 2007. Traffic. 8:1854-72. 6. Ornitz DM, Xu J, Colvin JS, et al. 1996. J Biol Chem. 271:15292-7. 7. Zhang X, Ibrahimi OA, Olsen SK, et al. 2006. J Biol Chem. 281:15694-700. |
Background |
Murine KGF-1 also known as Fibroblast growth factor 7 (FGF-7), is encoded by the FGF7 gene. KGF-1 only binds to the b splice form of the tyrosine kinase receptor, FGFR2b/KGFR. Affinity between KGF-1 and its receptor can be increased by heparin or heparan sulfate proteoglycan. FGF-10, also called keratinocyte growth factor 2 (KGF-2), shares 51 % amino acid sequence identity and similar function to KGF-1, but uses an additional receptor, FGFR2c. KGF-1 plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. KGF-1 actives on keratinocytes, and exhibits mitogenic activity for epidermal cells, but essentially no activity for fibroblasts. KGF-1 has species crossactive, murine KGF-1 shares 96 % amino acid sequence identity with human and rat. |
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