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카테고리
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Cytokines & Growth Factor
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CAT.NO
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LGP-12-057
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PRODUCT
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KGF-2/FGF-10, Mouse (Murine Keratinocyte Growth Factor-2/FGF-10)
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SIZE
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25ug, 100ug, 500ug
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PRICE
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KRW 285,000, 880,000, 2,569,000
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Technical Parameters
| Accession |
O35565 |
| GeneID |
14165 |
| Source |
Escherichia coli. |
| Molecular Weight |
Approximately19.5 kDa, a single non-glycosylated polypeptide chain containing 173 amino acids. |
| Quantity |
25µg/100µg/500µg |
| AA Sequence |
QALGQDMVSQ EATNCSSSSS SFSSPSSAGR HVRSYNHLQG DVRWRRLFSF TKYFLTIEKN GKVSGTKNED CPYSVLEITS VEIGVVAVKA INSNYYLAMN KKGKLYGSKE FNNDCKLKER IEENGYNTYA SFNWQHNGRQ MYVALNGKGA PRRGQKTRRK NTSAHFLPMT IQT |
| Purity |
> 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg. |
| Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 µm filtered concentrated solution in 2 × PBS, 600 mM NaCl, pH 7.4, 1 mM mercaptoethanol. |
| Endotoxin |
Less than 1 EU/µg of rMuKGF-2/FGF-10 as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Usage |
This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
| Reference |
1. Emoto H, Tagashira S, Mattei MG, et al. 1997. J Biol Chem. 272:23191-4.
2. Tagashira S, Harada H, Katsumata T, et al. 1997. Gene. 197:399-404.
3. Carninci P, Kasukawa T, Katayama S, et al. 2005. Science. 309:1559-63.
4. Igarashi M, Finch PW, Aaronson SA. 1998. J Biol Chem. 273:13230-5. |
| Background |
Fibroblast growth factor 10 belongs to the fibroblast growth factor (FGF) family, which is involved in a variety of biological processes such as embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. Like most other FGF family members, FGF-10 also has a heparin-binding domain and plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. In addition, FGF-10 may take parts in wound healing and is required for normal branching morphogenesis. Recombinant murine FGF-10 contains a 173 amino acids and it shares 94 % and 100 % a.a. sequence identity with human and rat FGF-10. |
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