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카테고리
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Cytokines & Growth Factor
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CAT.NO
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LGP-13-002
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PRODUCT
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IL-8/CXCL8, Canine (Interleukin-8/CXCL8)
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SIZE
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25ug, 100ug, 500ug
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PRICE
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KRW 285,000, 880,000, 2,569,000
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Technical Parameters
| Synonyms |
(Ser-IL-8)72, GCP/IL-8 protein I, IL8/NAP1 form III, LYNAP, MDNCF-c, NAF |
| Accession |
P41324 |
| GeneID |
403850 |
| Source |
Escherichia coli. |
| Molecular Weight |
Approximately 9.1 kDa, a single non-glycosylated polypeptide chain containing 79 amino acids. |
| Quantity |
25µg/100µg/500µg |
| AA Sequence |
AVLSRVSSEL RCQCIKTHST PFHPKYIKEL RVIDSGPHCE NSEIIVKLFN GNEVCLDPKE KWVQKVVQIF LKKAEKQDP |
| Purity |
> 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR2 transfected murine BaF3 cells is in a concentration range of 0.15-0.75 ng/ml. |
| Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4. |
| Endotoxin |
Less than 1 EU/μg of rCaIL-8/CXCL8 as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Usage |
This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
| SDS-PAGE |
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| Reference |
1. Modi WS, Dean M, Seuanez HN, et al. 1990. Hum Genet. 84:185-7.
2. Wolff B, Burns AR, Middleton J, et al. 1998. J Exp Med. 188:1757-62.
3. Utgaard JO, Jahnsen FL, Bakka A, et al. 1998. J Exp Med. 188:1751-6.
4. Van Damme J, Rampart M, Conings R, et al. 1990. Eur J Immunol. 20:2113-8. |
| Background |
Interleukin-8 (IL-8) is encoded by the IL8 gene andproduced by macrophages and other cell types such as epithelial cells. It is also synthesized by endothelial cells, which store IL-8 in their storage vesicles. There are many receptors capable to bind IL-8, the most affinity to IL-8 are receptors CXCR1, and CXCR2. As a member of the CXC chemokine family, function of IL-8 is the induction of chemotaxis in its target cells, like neutrophil granulocytes, basophils, and T-cells. . IL-8 is often associated with inflammation and has been cited as a proinflammatory mediator in gingivitis and psoriasis. |
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