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카테고리
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Cytokines & Growth Factor
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CAT.NO
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LGP-14-017
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PRODUCT
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IL-22, Rat (Interleukin-22)
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SIZE
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10ug, 100ug, 250ug
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PRICE
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KRW 285,000, 1,522,000, 2,622,000
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Technical Parameters
| Synonyms |
Cytokine Zcyto 18, TIF |
| Accession |
Q198B4 |
| Unigene |
Rn.195879. |
| Source |
Escherichia coli. |
| Molecular Weight |
Approximately16.6 kDa, a single non-glycosylated polypeptide chain containing 146 amino acids. |
| Quantity |
10µg/100µg/250µg |
| AA Sequence |
LPINSQCKLE AANFQQPYIV NRTFMLAKEA SLADNNTDVR LIGEELFRGV KAKDQCYLMK QVLNFTLEDV LLPQSDRFQP YMQEVVPFLT KLSIHLSPCH ISGDDQNIQK NVRQLKETVQ KLGESGEIKA IGELDLLFMS LRNACV |
| Purity |
> 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by inducing IL-10 secretion of human COLO 205 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg. |
| Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin |
Less than 1 EU/μg of rRtIL-22 as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Usage |
This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
| SDS-PAGE |
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| Reference |
1. Zeng G, Chen CY, Huang D, et al. 2011. J Immunol, 187: 190-9.
2. Cocco CandAiroldi I. 2012. Immunotherapy, 4: 668.
3. Honda K. 2012. Immunity, 37: 952-4.
4. Gurney AL. 2004. Int Immunopharmacol, 4: 669-77. |
| Background |
IL-22 is belonging to IL-10 family of regulatory cytokines which includes IL-10, IL-19, IL-20, IL-22, IL-24 and IL-26. Members of this family share partial homology in their amino acid sequences, but they are dissimilar in their biological functions. Produced by T lymphocytes and dendritic cells, IL-10 contributes to the inflammatory response in vivo. IL-22 signals through CRF2-4 and IL-22. It along with IL-17 is rapidly produced by splenic LTi-like cells and can be also produced by Th17 cells and likely plays a role in the coordinated response of both adaptive and innate immune systems. Recombinant rat Interleukin-22 contains 146 amino acids and it has approximately 79 % and 92 % amino acid sequence identity with human and murine IL-22. |
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