Ä«Å×°í¸®
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Cytokines & Growth Factor
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CAT.NO
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LGP-14-040
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PRODUCT
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FGF-21, Rat (Fibroblast Growth Factor 21)
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SIZE
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25ug, 100ug, 500ug
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PRICE
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KRW 285,000, 880,000, 2,569,000
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Technical Parameters
Accession |
Q8VI80 |
GeneID |
170580 |
Source |
Escherichia coli. |
Molecular Weight |
Approximately 19.7 kDa, a single non-glycosylated polypeptide chain containing 180 amino acids. |
Quantity |
25µg/100µg/500µg |
AA Sequence |
AYPISDSSPL LQFGGQVRQR YLYTDDDQDT EAHLEIREDG TVVGTAHRSP ESLLELKALK PGVIQILGVK ASRFLCQQPD GTLYGSPHFD PEACSFRELL LKDGYNVYQS EAHGLPLRLP QKDSQDPATR GPVRFLPMPG LPHEPQEQPG VLPPEPPDVG SSDPLSMVEP LQGRSPSYAS |
Purity |
> 95 % by SDS-PAGE and HPLC analyses. |
Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ¥ìg/ml, corresponding to a specific activity of > 2.0 ¡¿ 103 IU/mg in the presence of 5 µg/ml of rMuKlotho-¥â and 10 ¥ìg/ml of heparin. |
Physical Appearance |
White lyophilized powder. |
Formulation |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, 500 mM NaCl, pH 7.4. |
Endotoxin |
Less than 1 EU/µg of rRtFGF-21 as determined by LAL method. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ¡Â -20 ¡ÆC. Further dilutions should be made in appropriate buffered solutions. |
Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 ¡ÆC as supplied. - 1 month, 2 to 8 ¡ÆC under sterile conditions after reconstitution. - 3 months, -20 to -70 ¡ÆC under sterile conditions after reconstitution. |
Usage |
This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
SDS-PAGE |
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Reference |
1. Nishimura T, Nakatake Y, Konishi M, et al. 2000. Biochim Biophys Acta. 1492:203-6. 2. Kharitonenkov A, Shiyanova TL, Koester A, et al. 2005. J Clin Invest. 115:1627-35. 3. Kurosu H, Choi M, Ogawa Y, et al. 2007. J Biol Chem. 282:26687-95 |
Background |
Fibroblast growth factor 21 (FGF-21) encoded by the FGF-21 gene belongs to the large FGF family and it is specifically induced by HMGCS2 activity. In mice, brown adipose tissue becomes a source of systemic FGF-21 after cold exposure. FGF-21 stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression) and the activity depends on the presence of KLB. Recombinant rat FGF-21 contains 180 amino acids residues and show limited binding to heparin. In addition, rat FGF-21 respectively shows 81 % and 92 % a.a. identity to human and murine FGF-21, and it show activity on murine and human cells. |
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