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카테고리
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Cytokines & Growth Factor
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CAT.NO
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LGP-14-044
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PRODUCT
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HB-EGF, Rat (Heparin-binding EGF-like Growth Factor)
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SIZE
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50ug, 250ug, 1mg
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PRICE
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KRW 285,000, 760,000, 2,100,000
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Technical Parameters
| Synonyms |
HBEGF, DT-R |
| Accession |
Q06175 |
| GeneID |
25433 |
| Source |
Escherichia coli. |
| Molecular Weight |
Approximately 9.7 kDa, a single non-glycosylated polypeptide chain containing 86 amino acids. |
| Quantity |
50µg/250µg/1000µg |
| AA Sequence |
DLEGTDLDLF KVAFSSKPQA LATPGKEKNG KKKRKGKGLG KKRDPCLKKY KDYCIHGECR YLKELRIPSC HCLPGYHGQR CHGLTL |
| Purity |
> 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0 × 106 IU/mg. |
| Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, 300 mM NaCl, pH 7.4, 5 % trehalose. |
| Endotoxin |
Less than 1 EU/μg of rRtHB-EGF as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Usage |
This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
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| Reference |
1. Jin K, Mao XO, Sun Y, et al. 2002. J Neurosci. 22:5365-73.
2. Higashiyama S, Abraham JA, Miller J, et al. 1991. Science. 251:936-9. |
| Background |
Heparin-binding epidermal growth factor (HB-EGF)-like growth factor (EGF) is found in cerebral neurons. Its expression is increased after hypoxic or ischemic injury, which also stimulates neurogenesis. HB-EGF has been implicated as a participant in a variety of normal physiological processes such as blastocyst implantation and wound healing, and in pathological processes such as tumor growth, SMC hyperplasia and atherosclerosis. HB-EGF is an 87 amino acid mitogenic and chemotactic glycoprotein containing an EGF-like domain with six conserved cysteine residues. Rat HB-EGF shares about 76 % a.a. sequence identity with human HB-EGF. |
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첨부파일
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