Ä«Å×°í¸®
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Cytokines & Growth Factor
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CAT.NO
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LGP-15-005
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PRODUCT
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IL-8/CXCL8, Porcine (Interleukin-8/CXCL8)
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SIZE
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10ug, 100ug, 1mg
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PRICE
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krw 285,000, 505,000, 2,112,000
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Technical Parameters
Synonyms |
(Ser-IL-8)72, GCP/IL-8 Protein I, IL8/NAP1 form III, LYNAP, MDNCF-c, NAF |
Accession |
P26894 |
GeneID |
396880 |
Source |
Escherichia coli. |
Molecular Weight |
Approximately 9.1 kDa, a single non-glycosylated polypeptide chain containing 78 amino acids. |
Quantity |
10µg/100µg/1000µg |
AA Sequence |
ARVSAELRCQ CINTHSTPFH PKFIKELRVI ESGPHCENSE IIVKLVNGKE VCLDPKEKWV QKVVQIFLKR TEKQQQQQ |
Purity |
> 95 % by SDS-PAGE and HPLC analyses. |
Biological Activity |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration range of 20-200 ng/ml. |
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation |
Lyophilized from a 0.2 ¥ìm filtered concentrated solution in 2 ¡¿ PBS, pH 7.4. |
Endotoxin |
Less than 1 EU/¥ìg of rPoIL-8/CXCL8 as determined by LAL method. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ¡Â -20 ¡ÆC. Further dilutions should be made in appropriate buffered solutions. |
Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 ¡ÆC as supplied. - 1 month, 2 to 8 ¡ÆC under sterile conditions after reconstitution. - 3 months, -20 to -70 ¡ÆC under sterile conditions after reconstitution. |
Usage |
This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
SDS-PAGE |
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Reference |
1. Modi WS, Dean M, Seuanez HN, et al. 1990. Hum Genet. 84:185-7. 2. Wolff B, Burns AR, Middleton J, et al. 1998. J Exp Med. 188:1757-62. 3. Utgaard JO, Jahnsen FL, Bakka A, et al. 1998. J Exp Med. 188:1751-6. 4. Van Damme J, Rampart M, Conings R, et al. 1990. Eur J Immunol. 20:2113-8. |
Background |
Interleukin-8 (IL-8) is encoded by the IL8 gene and produced by macrophages and other cell types such as epithelial cells. It is also synthesized by endothelial cells, which store IL-8 in their storage vesicles. There are many receptors capable to bind IL-8, the most affinity to IL-8 are receptors CXCR1, and CXCR2. As a member of the CXC chemokine family, function of IL-8 is the induction of chemotaxis in its target cells, like neutrophil granulocytes, basophils, and T-cells. . IL-8 is often associated with inflammation and has been cited as a proinflammatory mediator in gingivitis and psoriasis. |
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