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카테고리 Cytokines & Growth Factor
CAT.NO LGP-15-008
PRODUCT IL-2 Cys141Ser, Equine (Interleukin-2 Cys141Ser)
SIZE 10ug, 100ug, 500ug
PRICE KRW 285,000, 1,137,000, 3,330,000
Technical Parameters
Accession P37997
GeneID 100034204
Source Escherichia coli.
Molecular Weight Approximately 14.9 kDa, a single non-glycosylated polypeptide chain containing 129 amino acids.
Quantity 10µg/100µg/500µg
AA Sequence APTSSSKRET QQQLKQLQMD LKLLLEGVNN NKNPKLSKML TFKINMPKKA TELKHLQCLE EELKPLEEML KNFLSKDIKE LMSNINVTVL GLKGSETRFT CEYDDETGTI VEFLNKWITF SQSIFSTMT
Purity > 97 % by SDS-PAGE and HPLC analyses.
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 1.0 μg/ml, corresponding to a specific activity of > 1000 IU/mg.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 6.5.
Endotoxin Less than 0.1 EU/µg of rEqIL-2 Cys141Ser as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Usage This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
SDS-PAGE
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