|
카테고리
|
Chemokines
|
|
CAT.NO
|
LGP-20-006
|
|
PRODUCT
|
ENA-78(8-78 a.a.)/CXCL5, Human (Epithelial Neutrophil Activating Peptide-78, 8-78 a.a./CXCL5)
|
|
SIZE
|
20ug, 100ug, 500ug
|
|
PRICE
|
KRW 285,000, 880,000, 2,569,000
|
Technical Parameters
| Synonyms |
CXCL5, Small-inducible cytokine B5 |
| Species |
9 |
| Accession |
P42830 |
| GeneID |
6374 |
| Source |
Escherichia coli. |
| Molecular Weight |
Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 71 amino acids. |
| Quantity |
20µg/100µg/500µg |
| AA Sequence |
LRELRCVCLQ TTQGVHPKMI SNLQVFAIGP QCSKVEVVAS LKNGKEICLD PEAPFLKKVI QKILDGGNKE N |
| Purity |
> 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration of 10.0-100.0 ng/ml. |
| Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4. |
| Endotoxin |
Less than 1 EU/μg of rHuENA-78, 8 - 78 a.a./CXCL5 as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Usage |
This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
| Reference |
1. Chang MS, McNinch J, Basu R, et al. 1994. J Biol Chem. 269:25277-82.
2. O'Donovan N, Galvin M, Morgan JG. 1999. Cytogenet Cell Genet. 84:39-42.
3. Persson T, Monsef N, Andersson P, et al. 2003. Clin Exp Allergy. 33:531-7.
4. Wuyts A, Govaerts C, Struyf S, et al. 1999. Eur J Biochem. 260:421-9. |
| Background |
CXCL5 is a member of the CXC chemokine family and also known as epithelial-derived neutrophil-activating peptide 78 (ENA-78). It is produced following stimulation of cells with the inflammatory cytokines interleukin-1 or tumor necrosis factor-alpha. In vitro, ENA-78 (8-78) and ENA-78 (9-78) show a threefold higher chemotactic activity for neutrophil granulocytes. They are produced by proteolytic cleavage after secretion from peripheral blood monocytes. Recombinant human CXCL5 (8-78 a.a.) contains 71 amino acids which is a single non-glycosylated polypeptide chain. Human CXCL5 shares 57 % amino acid sequence identity with mouse and rat CXCL5. |
|
|
첨부파일
|
|
|
|
|
|
