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카테고리
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Chemokines
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CAT.NO
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LGP-20-012
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PRODUCT
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SDF-1α/CXCL12α, Human (Stromal-Cell Derived Factor-1 alpha/CXCL12 alpha)
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SIZE
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10ug, 100ug, 500ug
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PRICE
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KRW 285,000, 880,000, 2,569,000
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Technical Parameters
| Synonyms |
SDF-1 alpha, hSDF-1 alpha, IRH, hIRH, PBSF |
| Species |
9 |
| Accession |
P48061 |
| GeneID |
6387 |
| Source |
Escherichia coli. |
| Molecular Weight |
Approximately 8.0 kDa, a single non-glycosylated polypeptide chain containing 68 amino acids. |
| Quantity |
10µg/100µg/500µg |
| AA Sequence |
KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK |
| Purity |
> 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using PHA and rHuIL-2 activated human peripheral blood T-lymphocytes is in a concentration range of 20-80 ng/ml. |
| Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB pH 7.0, 130 mM NaCl. |
| Endotoxin |
Less than 1 EU/μg of rHuSDF-1α/CXCL12α as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Usage |
This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
| Reference |
1. Shirozu M, Nakano T, Inazawa J, et al. 1995. Genomics. 28:495-500.
2. Yu L, Cecil J, Peng SB, et al. 2006. Gene. 374:174-9.
3. De La Luz Sierra M, Yang F, Narazaki M, et al. 2004. Blood. 103:2452-9.
4. Charnaux N, Brule S, Hamon M, et al. 2005. FEBS J. 272:1937-51.
5. Bleul CC, Fuhlbrigge RC, Casasnovas JM, et al. 1996. J Exp Med. 184:1101-9.
6. Ara T, Nakamura Y, Egawa T, et al. 2003. Proc Natl Acad Sci U S A. 100:5319-23.
7. Askari AT, Unzek S, Popovic ZB, et al. 2003. Lancet. 362:697-703.
8. Ma Q, Jones D, Borghesani PR, et al. 1998. Proc Natl Acad Sci U S A. 95:9448-53.
9. Kryczek I, Wei S, Keller E, et al. 2007. Am J Physiol Cell Physiol. 292:C987-95. |
| Background |
CXCL12 also known as SDF-1 is belonging to the CXC chemokine family. It is encoded by the CXCL12 gene. In recently study, Human CXCL12 is expressed as six isoforms that differ only in the C-terminal tail. And all SDF-1 isoforms undergo proteolytic processing of the first two N-terminal amino acids. Contrast to the canonical sequence SDF-1β, SDF-1α is shorter by four amino acids at the C-terminal tail. On the cell surface, the receptor for this chemokine is CXCR4 and syndecan4. CXCL12 is strongly chemotactic for T-lymphocytes, monocytes, but not neutrophils. CXCL12 is a very important factor in carcinogenesis and the neovascularisation linked to tumor progression. |
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