Ä«Å×°í¸®
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Chemokines
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CAT.NO
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LGP-20-019
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PRODUCT
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I-309/CCL1, Human
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SIZE
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10ug, 100ug, 500ug
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PRICE
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KRW 285,000, 880,000, 2,569,000
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Technical Parameters
Synonyms |
Small-inducible cytokine A1, T lymphocyte-secreted protein I-309 |
Species |
9 |
Accession |
P22362 |
GeneID |
6346 |
Source |
Escherichia coli. |
Molecular Weight |
Approximately 8.6 kDa, a single, non-glycosylated polypeptide chain containing 74 amino acids. |
Quantity |
10µg/100µg/500µg |
AA Sequence |
SKSMQVPFSR CCFSFAEQEI PLRAILCYRN TSSICSNEGL IFKLKRGKEA CALDTVGWVQ RHRKMLRHCP SKRK |
Purity |
>97% by SDS-PAGE and HPLC analyses. |
Biological Activity |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 10-100 ng/ml. |
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation |
Lyophilized from a 0.2 ¥ìm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl. |
Endotoxin |
Less than 1 EU/¥ìg of rHuI-309/CCL1 as determined by LAL method. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ¡Â -20 ¡ÆC. Further dilutions should be made in appropriate buffered solutions. |
Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 ¡ÆC as supplied. - 1 month, 2 to 8 ¡ÆC under sterile conditions after reconstitution. - 3 months, -20 to -70 ¡ÆC under sterile conditions after reconstitution. |
Usage |
This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
SDS-PAGE |
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Reference |
1. Miller MD, Krangel MS. 1992. Proc Natl Acad Sci U S A. 89:2950-4. 2. Roos RS, Loetscher M, Legler DF, et al. 1997. J Biol Chem. 272:17251-4. 3. Miller MD, Wilson SD, Dorf ME, et al. 1990. J Immunol. 145:2737-44. |
Background |
Chemokine (C-C motif) ligand 1 (CCL1) belongs to a family inflammatory cytokines and also known as chemokines. It is a small glycoprotein secreted by activated T cells with a molecular weight of approximately 8.5 kDa. CCL1 attracts monocytes, NK cells, and immature B cells and dendritic cells by interacting with a cell surface chemokine receptor called CCR8. Human CCL1 has been assumed to be a homologue of the mouse TCA3. While the two proteins share only approximately 42 % amino acid sequence identity, both chemokines contain an extra pair of cysteine residues not found in most other chemokines. |
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