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Ä«Å×°í¸® Chemokines
CAT.NO LGP-20-022
PRODUCT LD78¥â/CCL3L1, Human
SIZE 20ug, 100ug, 500ug
PRICE KRW 285,000, 880,000, 2,569,000
Technical Parameters
Synonyms G0/G1 switch regulatory protein 19-2, LD78-beta (1-70), PAT 464.2, Small-inducible cytokine A3-like 1, Tonsillar lymphocyte LD78 beta protein
Accession P16619
GeneID 6349
Source Escherichia coli.
Molecular Weight Approximately 7.8 kDa protein containing 70 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.
Quantity 20µg/100µg/500µg
AA Sequence APLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPSV IFLTKRGRQV CADPSEEWVQ KYVSDLELSA
Purity > 97 % by SDS-PAGE and HPLC analyses.
Biological Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 1.0-10 ng/ml.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 ¥ìm filtered concentrated solution in PBS, pH 7.4.
Endotoxin Less than 1 EU/¥ìg of rHuLD78-¥â/CCL3L1 as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ¡Â -20 ¡ÆC. Further dilutions should be made in appropriate buffered solutions.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 ¡ÆC as supplied.
- 1 month, 2 to 8 ¡ÆC under sterile conditions after reconstitution.
- 3 months, -20 to -70 ¡ÆC under sterile conditions after reconstitution.
Usage This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Reference 1. Irving SG, Zipfel PF, Balke J, et al. 1990. Nucleic Acids Res. 18:3261-70.
2. Hirashima M, Ono T, Nakao M, et al. 1992. DNA Seq. 3:203-12.
3. Miyamoto Y, Machida K, Mizunuma M, et al. 2002. J Biol Chem. 277:28810-4.
4. Struyf S, Menten P, Lenaerts JP, et al. 2001. Eur J Immunol. 31:2170-8.
Background CCL3L1, also named LD78-beta, is belonging to the intercrine beta (chemokine CC) family and it is encoded by the CCL3L1 gene in humans. This protein binds to several chemokine receptors including chemokine binding protein 2 (CCBP2 or D6) and chemokine (C-C motif) receptor 5 (CCR5). It is an inhibitor of HIV-1-infection and chemotactic for lymphocytes and monocytes. Recombinant human LD78¥â is a 7.7 kDa protein containing 70 amino acid residues, including the four conserved cysteine residues present in CC chemokines.
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