Ä«Å×°í¸®
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Chemokines
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CAT.NO
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LGP-20-022
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PRODUCT
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LD78¥â/CCL3L1, Human
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SIZE
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20ug, 100ug, 500ug
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PRICE
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KRW 285,000, 880,000, 2,569,000
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Technical Parameters
Synonyms |
G0/G1 switch regulatory protein 19-2, LD78-beta (1-70), PAT 464.2, Small-inducible cytokine A3-like 1, Tonsillar lymphocyte LD78 beta protein |
Accession |
P16619 |
GeneID |
6349 |
Source |
Escherichia coli. |
Molecular Weight |
Approximately 7.8 kDa protein containing 70 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines. |
Quantity |
20µg/100µg/500µg |
AA Sequence |
APLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPSV IFLTKRGRQV CADPSEEWVQ KYVSDLELSA |
Purity |
> 97 % by SDS-PAGE and HPLC analyses. |
Biological Activity |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 1.0-10 ng/ml. |
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation |
Lyophilized from a 0.2 ¥ìm filtered concentrated solution in PBS, pH 7.4. |
Endotoxin |
Less than 1 EU/¥ìg of rHuLD78-¥â/CCL3L1 as determined by LAL method. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ¡Â -20 ¡ÆC. Further dilutions should be made in appropriate buffered solutions. |
Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 ¡ÆC as supplied. - 1 month, 2 to 8 ¡ÆC under sterile conditions after reconstitution. - 3 months, -20 to -70 ¡ÆC under sterile conditions after reconstitution. |
Usage |
This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
Reference |
1. Irving SG, Zipfel PF, Balke J, et al. 1990. Nucleic Acids Res. 18:3261-70. 2. Hirashima M, Ono T, Nakao M, et al. 1992. DNA Seq. 3:203-12. 3. Miyamoto Y, Machida K, Mizunuma M, et al. 2002. J Biol Chem. 277:28810-4. 4. Struyf S, Menten P, Lenaerts JP, et al. 2001. Eur J Immunol. 31:2170-8. |
Background |
CCL3L1, also named LD78-beta, is belonging to the intercrine beta (chemokine CC) family and it is encoded by the CCL3L1 gene in humans. This protein binds to several chemokine receptors including chemokine binding protein 2 (CCBP2 or D6) and chemokine (C-C motif) receptor 5 (CCR5). It is an inhibitor of HIV-1-infection and chemotactic for lymphocytes and monocytes. Recombinant human LD78¥â is a 7.7 kDa protein containing 70 amino acid residues, including the four conserved cysteine residues present in CC chemokines. |
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