Ä«Å×°í¸®
|
Chemokines
|
CAT.NO
|
LGP-20-026
|
PRODUCT
|
MCP-3/CCL7, Human (Chemotactic Protein-3/CCL7)
|
SIZE
|
10ug, 100ug, 500ug
|
PRICE
|
KRW 285,000, 880,000, 2,569,000
|
Technical Parameters
Synonyms |
NC28, Small-inducible cytokine A7 |
Species |
9 |
Accession |
P80098 |
GeneID |
6354 |
Source |
Escherichia coli. |
Molecular Weight |
Approximately 9.0 kDa, a single, non-glycosylated polypeptide chain containing 76 amino acids. |
Quantity |
10µg/100µg/500µg |
AA Sequence |
QPVGINTSTT CCYRFINKKI PKQRLESYRR TTSSHCPREA VIFKTKLDKE ICADPTQKWV QDFMKHLDKK TQTPKL |
Purity |
> 97 % by SDS-PAGE and HPLC analyses. |
Biological Activity |
Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/ml. |
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation |
Lyophilized from a 0.2 ¥ìm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl. |
Endotoxin |
Less than 1 EU/¥ìg of rHuMCP-3/CCL7 as determined by LAL method. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ¡Â -20 ¡ÆC. Further dilutions should be made in appropriate buffered solutions. |
Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 ¡ÆC as supplied. - 1 month, 2 to 8 ¡ÆC under sterile conditions after reconstitution. - 3 months, -20 to -70 ¡ÆC under sterile conditions after reconstitution. |
Usage |
This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
SDS-PAGE |
|
Reference |
1. Opdenakker G, Fiten P, Nys G, et al. 1994. Genomics. 21:403-8. 2. Opdenakker G, Froyen G, Fiten P, et al. 1993. Biochem Biophys Res Commun. 191:535-42. |
Background |
Human CCL7, also known as monocyte chemotactic protein 3 (MCP-3), is belonging to the CC chemokine family. MCP-3 has two homogeneous MCP-1 (CCL2) and MCP-2 (CCL8). These three MCPs were found by IL-1-beta triggered human MG-63 osteosarcoma cells. CCL7 shares 71 % and 58 % a.a. sequence identity with MCP-1 and MCP-2, respectively. CCL7 has chemotactic function for monocytes and eosinophils, but not for neutrophils. In addition, it can also chemoattract activated NK cells. |
|
÷ºÎÆÄÀÏ
|
|
|
|
|