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카테고리
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Chemokines
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CAT.NO
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LGP-20-029
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PRODUCT
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MCP-4/CCL13, Human (Monocyte Chemotactic Protein-4/CCL13)
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SIZE
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20ug, 100ug, 500ug
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PRICE
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KRW 285,000, 880,000, 2,569,000
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Technical Parameters
| Synonyms |
CK-beta-10, NCC-1, Small-inducible cytokine A13 |
| Species |
9 |
| Accession |
Q99616 |
| GeneID |
6357 |
| Source |
Escherichia coli. |
| Molecular Weight |
Approximately 8.6 kDa, a single non-glycosylated polypeptide chain containing 75 amino acids. |
| Quantity |
20µg/100µg/500µg |
| AA Sequence |
QPDALNVPST CCFTFSSKKI SLQRLKSYVI TTSRCPQKAV IFRTKLGKEI CADPKEKWVQ NYMKHLGRKA HTLKT |
| Purity |
> 96 % by SDS-PAGE and HPLC analyses. |
| Biological Activity |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration of 10-100 ng/ml. |
| Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 130 mM NaCl. |
| Endotoxin |
Less than 1 EU/μg of rHuMCP-4/CCL13 as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Usage |
This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
| Reference |
1. Uguccioni M, Loetscher P, Forssmann U, et al. 1996. J Exp Med. 183:2379-84.
2. Berkhout TA, Sarau HM, Moores K, et al. 1997. J Biol Chem. 272:16404-13.
3. Garcia-Zepeda EA, Combadiere C, Rothenberg ME, et al. 1996. J Immunol. 157:5613-26. |
| Background |
Human CCL13 is belonging to the CC chemokine family and is encoded by the gene CCL13 in humans. CCL13 (MCP-4) shares 56-61 % sequence identity with MCP-1 (CCL2) and MCP-3 (CCL7) and is 60 % identical to Eotaxin (CCL11). CCL13 was a potent chemoattractant for monocytes and eosinophils and stimulated histamine release from basophils. CCL13 can induce a calcium flux in HEK-293 cells transfected with the receptor CCR2B and CCR3. That shows the function receptors of CCL3 are CCR2B and CCR3. |
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