Ä«Å×°í¸®
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Chemokines
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CAT.NO
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LGP-20-030
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PRODUCT
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HCC-1/CCL14, Human (Hemofiltrate CC Chemokine-1/CCL14)
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SIZE
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10ug, 100ug, 500ug
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PRICE
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KRW 285,000, 880,000, 2,569,000
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Technical Parameters
Synonyms |
HCC-1/HCC-3, NCC-2, Small-inducible Cytokine A14 |
Species |
9 |
Accession |
Q16627 |
GeneID |
6358 |
Source |
Escherichia coli. |
Molecular Weight |
Approximately 8.4 kDa, a single non-glycosylated polypeptide chain containing 72 amino acids. |
Quantity |
10µg/100µg/500µg |
AA Sequence |
TESSSRGPYH PSECCFTYTT YKIPRQRIMD YYETNSQCSK PGIVFITKRG HSVCTNPSDK WVQDYIKDMK EN |
Purity |
> 96 % by SDS-PAGE and HPLC analyses. |
Biological Activity |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration of 5.0-20 ng/ml. |
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation |
Lyophilized from a 0.2 ¥ìm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl. |
Endotoxin |
Less than 1 EU/¥ìg of rHuHCC-1/CCL14 as determined by LAL method. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ¡Â -20 ¡ÆC. Further dilutions should be made in appropriate buffered solutions. |
Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 ¡ÆC as supplied. - 1 month, 2 to 8 ¡ÆC under sterile conditions after reconstitution. - 3 months, -20 to -70 ¡ÆC under sterile conditions after reconstitution. |
Usage |
This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
Reference |
1. Schulz-Knappe P, Magert HJ, Dewald B, et al. 1996. J Exp Med. 183:295-9. 2. Detheux M, Standker L, Vakili J, et al. 2000. J Exp Med. 192:1501-8. 3. Pardigol A, Forssmann U, Zucht HD, et al. 1998. Proc Natl Acad Sci U S A. 95:6308-13. |
Background |
Human CCL14 is belonging to the CC chemokine family. It has two isoforms, CCL14a (HCC-1) and CCL14b (HCC-3). The sequence of HCC-3 differs from HCC-1 as follow: 27-27 R¡æ QTGGKPKVVKIQLKLVG. CCL14 was first isolated from the hemofiltrate of human patients with chronic renal failure. CCL14 promotes chemotaxis of T lymphocytes, monocytes and eosinophils, and inhibits infection of M-tropic human immunodeficiency virus type 1 and is a ligand for CCR1, CCR3 and CCR5. CCL14 is expressed constitutively in several normal tissues: spleen, liver, skeletal and heart muscle, gut, and bone marrow, present at high concentrations in plasma. |
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