Ä«Å×°í¸®
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Chemokines
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CAT.NO
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LGP-20-036
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PRODUCT
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MIP-4/CCL18, Human (Macrophage Inflammatory Protein-4/CCL18)
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SIZE
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10ug, 100ug, 500ug
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PRICE
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KRW 285,000, 880,000, 2,569,000
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.
Technical Parameters
Synonyms |
AMAC-1, PARC, DC-CK1 |
Species |
9 |
Accession |
P55774 |
GeneID |
6362 |
Source |
Escherichia coli. |
Molecular Weight |
Approximately 7.9 kDa, a single non-glycosylated polypeptide chain containing 69 amino acids. |
Quantity |
10µg/100µg/500µg |
AA Sequence |
AQVGTNKELC CLVYTSWQIP QKFIVDYSET SPQCPKPGVI LLTKRGRQIC ADPNKKWVQK YISDLKLNA |
Purity |
> 97 % by SDS-PAGE and HPLC analyses. |
Biological Activity |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration of 1.0-10 ng/ml. |
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation |
Lyophilized from a 0.2 ¥ìm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl. |
Endotoxin |
Less than 1 EU/¥ìg of rHuMIP-4/CCL18 as determined by LAL method. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ¡Â -20 ¡ÆC. Further dilutions should be made in appropriate buffered solutions. |
Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 ¡ÆC as supplied. - 1 month, 2 to 8 ¡ÆC under sterile conditions after reconstitution. - 3 months, -20 to -70 ¡ÆC under sterile conditions after reconstitution. |
Usage |
This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
SDS-PAGE |
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Reference |
1. Hieshima K, Imai T, Baba M, et al. 1997. J Immunol, 159: 1140-9. 2. Chenivesse C, Chang Y, Azzaoui I, et al. 2012. J Immunol, 189: 128-37. 3. Azzaoui I, Yahia SA, Chang Y, et al. 2011. Blood, 118: 3549-58. 4. Adema GJ, Hartgers F, Verstraten R, et al. 1997. Nature, 387: 713-7. |
Background |
Human CCL18 is encoded by the CCL18 gene located on the chromosome 17. As also named MIP-4, it shares 61 % sequence identity to human MIP-1¥á. CCL18 is mainly expressed by lung and some lymphoid tissues like lymph nodes express CCL18 at low level. It is chemotactic for both activated (CD3+) T cells and nonactivated (CD14-) lymphocytes, but not for monocytes or granulocytes. Involved in B-cell migration into B-cell follicles in lymph nodes. CCL18 plays a role in both humoral and cell-mediated immunity responses. |
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