Ä«Å×°í¸®
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Chemokines
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CAT.NO
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LGP-20-039
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PRODUCT
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MDC/CCL22, Human (Macrophage-Derived Chemokine/CCL22)
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SIZE
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20ug, 100ug, 500ug
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PRICE
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KRW 285,000, 880,000, 2,569,000
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Technical Parameters
Synonyms |
STCP-1, MDC (1-69), Macrophage-derived Chemokine |
Species |
9 |
Accession |
O00626 |
GeneID |
6367 |
Source |
Escherichia coli. |
Molecular Weight |
Approximately 8.1 kDa, a single, non-glycosylated polypeptide chain containing 69 amino acids. |
Quantity |
20µg/100µg/500µg |
AA Sequence |
GPYGANMEDS VCCRDYVRYR LPLRVVKHFY WTSDSCPRPG VVLLTFRDKE ICADPRVPWV KMILNKLSQ |
Purity |
> 97 % by SDS-PAGE and HPLC analyses. |
Biological Activity |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 10-100 ng/ml. |
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation |
Lyophilized from a 0.2 ¥ìm filtered concentrated solution in 20 mM PB, pH7.4, 500 mM NaCl. |
Endotoxin |
Less than 1 EU/¥ìg of rHuMDC/CCL22 as determined by LAL method. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ¡Â -20 ¡ÆC. Further dilutions should be made in appropriate buffered solutions. |
Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 ¡ÆC as supplied. - 1 month, 2 to 8 ¡ÆC under sterile conditions after reconstitution. - 3 months, -20 to -70 ¡ÆC under sterile conditions after reconstitution. |
Usage |
This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
SDS-PAGE |
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Reference |
1. Nomiyama H, Imai T, Kusuda J, et al. 1998. Cytogenet Cell Genet, 81: 10-1. 2. Godiska R, Chantry D, Raport CJ, et al. 1997. J Exp Med, 185: 1595-604. 3. Yamashita UandKuroda E. 2002. Crit Rev Immunol, 22: 105-14. 4. Katou F, Ohtani H, Nakayama T, et al. 2001. Am J Pathol, 158: 1263-70. |
Background |
CCL22 is a protein that in humans is encoded by the CCL22 gene, which locates on the Chr. 16. The protein is highly expressed in macrophage, monocyte-derived dendritic cell and thymus, additionally, also detected in the tissues of thymus, lymph node and appendix. CCL22 can bind to CCR4, and is a chemoattractant for monocytes, monocyte-derived dendritic cells, and natural killer cells, but not for neutrophils, eosinophils, and resting T-lymphocytes. After secreted from monocyte-derived dendritic cells, the protein can be proteolytic cleaved into three forms: MDC (3-69), MDC (5-69), MDC (7-69). |
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