Ä«Å×°í¸®
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Chemokines
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CAT.NO
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LGP-21-001
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PRODUCT
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IP-10/CXCL10, Rhesus (Macaque gamma-Interferon Inducible Protein 10/CXCL10)
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SIZE
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25ug, 100ug, 500ug
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PRICE
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KRW 285,000, 880,000, 2,569,000
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Technical Parameters
Synonyms |
Gamma-IP10, Small-inducible Cytokine B10 |
Accession |
Q8MIZ1 |
GeneID |
574243 |
Source |
Escherichia coli. |
Molecular Weight |
Approximately 8.7 kDa, a single non-glycosylated polypeptide chain containing 77 amino acids. |
Quantity |
25µg/100µg/500µg |
AA Sequence |
IPLSRTVRCT CISISNQPVN PRSLEKLEII PPSQFCPHVE IIATMKKKGE KRCLNPESKA IKNLLKAVSK ERSKRSP |
Purity |
> 97 % by SDS-PAGE and HPLC analyses. |
Biological Activity |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood T-lymphocytes is in a concentration range of 10-50 ng/ml. |
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4. |
Endotoxin |
Less than 1 EU/µg of rRhIP-10/CXCL10 as determined by LAL method. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ¡Â -20 ¡ÆC. Further dilutions should be made in appropriate buffered solutions. |
Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 ¡ÆC as supplied. - 1 month, 2 to 8 ¡ÆC under sterile conditions after reconstitution. - 3 months, -20 to -70 ¡ÆC under sterile conditions after reconstitution. |
Usage |
This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
SDS-PAGE |
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Reference |
1. Luster AD, Unkeless JC, Ravetch JV. 1985. Nature. 315:672-6. 2. Luster AD, Jhanwar SC, Chaganti RS, et al. 1987. Proc Natl Acad Sci U S A. 84:2868-71. 3. Booth V, Keizer DW, Kamphuis MB, et al. 2002. Biochemistry. 41:10418-25. 4. Dufour JH, Dziejman M, Liu MT, et al. 2002. J Immunol. 168:3195-204. 5. Angiolillo AL, Sgadari C, Taub DD, et al. 1995. J Exp Med. 182:155-62. 6. Dinarello CA. 1994. FASEB J. 8:1314-25. |
Background |
CXCL10 also known as IP-10 is belonging to the CXC chemokine family. It is encoded by the CXCL10 gene. CXCL10 was originally identified in monocytes, endothelial cells and fibroblasts as a responsor to IFN-¥ã. This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3. CXCL10 has been shown to be a chemoattractant for activated T-lymphocytes and monocytes/macrophages. It also has other roles, such as promotion of T cell adhesion to endothelial cells, and inhibition of bone marrow colony formation and angiogenesis. The rhesus macaque IP-10 shares 69 %, 95 %, 74 % a.a. sequence identity with human, murine, rat IP-10. |
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