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카테고리
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Chemokines
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CAT.NO
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LGP-21-003
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PRODUCT
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SAA1, Rhesus (Macaque Serum Amyloid A1)
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SIZE
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10ug, 100ug, 500ug
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PRICE
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KRW 285,000, 1,595,000, 4,442,000
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Technical Parameters
| Synonyms |
Amyloid fibril protein AA |
| Accession |
F6V9N7 |
| GeneID |
694827 |
| Source |
Escherichia coli. |
| Molecular Weight |
Approximately 11.7 kDa, a single non-glycosylated polypeptide chain containing 104 amino acids. |
| Quantity |
10µg/100µg/500µg |
| AA Sequence |
RSWFSFLGEA YDGARDMWRA YSDMKEANYK NSDKYFHARG NYDAAQRGPG GVWAAEVISD ARENIQKLLG RGAEDTLADQ AANEWGRSGK DPNHFRPAGL PEKY |
| Purity |
> 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/ml. |
| Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin |
Less than 1 EU/μg of rRhSAA1 as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Usage |
This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
| SDS-PAGE |
 |
| Reference |
1. Stevens G, Ramsay M, Kluve-Beckerman B, et al. 1993. Genomics, 15: 242-3.
2. Kluve-Beckerman BandSong M. 1995. Gene, 159: 289-90.
3. Kluve-Beckerman B, Malle E, Vitt H, et al. 1991. Biochem Biophys Res Commun, 181: 1097-102.
4. Milan E, Lazzari C, Anand S, et al. 2012. J Proteomics, 76 Spec No.: 91-101. |
| Background |
Serum Amyloid A protein-1 belongs to the SAA family of apolipoproteins that circulates in association with high-density lipoproteins (HDL). It is a multifunctional protein produced by hepatocytes in response to pro-inflammatory cytokines. SAA1 is a ligand for CD36/SR-B3, SR-B1, FPRL1, TLR2, and RAGE on monocytes/macrophages, inducing chemotaxis and generation of cytokines and tissue factor. It also can bind the surface of invading gram-negative bacteria, acting as an opsonin to aid clearance by macrophages. Additionally, SAA1 binds platelets, probably by engaging fibrinogen on the platelet surface. |
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첨부파일
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