Ä«Å×°í¸®
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Chemokines
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CAT.NO
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LGP-22-010
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PRODUCT
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SDF-1¥á/CXCL12¥á, Mouse (Murine Stromal-Cell Derived Factor-1 alpha/CXCL12¥á)
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SIZE
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10ug, 100ug, 500ug
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PRICE
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KRW 285,000, 880,000, 2,569,000
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Technical Parameters
Synonyms |
TPAR1, PBSF, TLSF |
Accession |
P40224 |
GeneID |
20315 |
Source |
Escherichia coli. |
Molecular Weight |
Approximately 8.0 kDa, a single non-glycosylated polypeptide chain containing 68 amino acids. |
Quantity |
10µg/100µg/500µg |
AA Sequence |
KPVSLSYRCP CRFFESHIAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK |
Purity |
> 97 % by SDS-PAGE and HPLC analyses. |
Biological Activity |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 50-100 ng/ml. |
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation |
Lyophilized from a 0.2 ¥ìm filtered concentrated solution in PBS, pH 7.4. |
Endotoxin |
Less than 1 EU/¥ìg of rMuSDF-1¥á/CXCL12¥á as determined by LAL method. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ¡Â -20 ¡ÆC. Further dilutions should be made in appropriate buffered solutions. |
Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 ¡ÆC as supplied. - 1 month, 2 to 8 ¡ÆC under sterile conditions after reconstitution. - 3 months, -20 to -70 ¡ÆC under sterile conditions after reconstitution. |
Usage |
This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
Reference |
1. Shirozu M, Nakano T, Inazawa J, et al. 1995. Genomics. 28:495-500. 2. De La Luz Sierra M, Yang F, Narazaki M, et al. 2004. Blood. 103:2452-9. 3. Charnaux N, Brule S, Hamon M, et al. 2005. FEBS J. 272:1937-51. 4. Bleul CC, Fuhlbrigge RC, Casasnovas JM, et al. 1996. J Exp Med. 184:1101-9. 5. Ara T, Nakamura Y, Egawa T, et al. 2003. Proc Natl Acad Sci U S A. 100:5319-23. 6. Askari AT, Unzek S, Popovic ZB, et al. 2003. Lancet. 362:697-703. 7. Ma Q, Jones D, Borghesani PR, et al. 1998. Proc Natl Acad Sci U S A. 95:9448-53. |
Background |
CXCL12 also known as SDF-1 is belonging to the CXC chemokine family. It is encoded by the CXCL12 gene. Murine CXCL12 is expressed as two isoforms that differ only in the C-terminal tail. And both SDF-1 isoforms undergo proteolytic processing of the first two N-terminal amino acids. Contrast to SDF-1¥â, SDF-1¥á is shorter by four amino acids at the C-terminal tail. On the cell surface, the receptor for this chemokine is CXCR4 and syndecan4. CXCL12 is strongly chemotactic for T-lymphocytes, monocytes, but not neutrophils. SDF-1 is highly conserved between species, murine CXCL12¥á shares approximately 93% amino acid sequence identity with human CXCL12¥á. |
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