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Ä«Å×°í¸® Enzymes & Inhibitors
CAT.NO LGP-40-012
PRODUCT Tissue Inhibitor of Metalloproteinases 2(TIMP-2, Human)
SIZE 10ug, 100ug, 500ug
PRICE KRW 285,000, 1,522,000, 4,440,000
Technical Parameters
Accession P16035
GeneID 7077
Source Escherichia coli.
Molecular Weight Approximately 21.8 kDa, a single non-glycosylated polypeptide chain containing 194 amino acids.
Quantity 10µg/100µg/500µg
AA Sequence CSCSPVHPQQ AFCNADVVIR AKAVSEKEVD SGNDIYGNPI KRIQYEIKQI KMFKGPEKDI EFIYTAPSSA VCGVSLDVGG KKEYLIAGKA EGDGKMHITL CDFIVPWDTL STTQKKSLNH RYQMGCECKI TRCPMIPCYI SSPDECLWMD WVTEKNINGH QAKFFACIKR SDGSCAWYRG AAPPKQEFLD IEDP
Purity > 95 % by SDS-PAGE and HPLC analyses.
Biological Activity Fully biologically active when compared to standard. The biologically active as determined by its ability to inhibit human MMP-2 cleavage of a fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4, with 3 % trehalose.
Endotoxin Less than 1 EU/µg of rHuTIMP-2 as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ¡Â -20 ¡ÆC. Further dilutions should be made in appropriate buffered solutions.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 ¡ÆC as supplied.
- 1 month, 2 to 8 ¡ÆC under sterile conditions after reconstitution.
- 3 months, -20 to -70 ¡ÆC under sterile conditions after reconstitution.
Usage This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
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