|
카테고리
|
Others
|
|
CAT.NO
|
LGP-60-009
|
|
PRODUCT
|
Retinoblastoma-Associated Protein Fragment, 137a.a. with His-tag(Rb137, Human, His)
|
|
SIZE
|
50ug, 250ug, 1mg
|
|
PRICE
|
KRW 285,000, 1,000,000, 3,300,000
|
Technical Parameters
| Synonyms |
p105-Rb, pRb, pp110 |
| Source |
Escherichia coli. |
| Molecular Weight |
Approximately 16.5 kDa, a single non-glycosylated polypeptide chain containing 146 amino acids. |
| Quantity |
50µg/250µg/1000µg |
| AA Sequence |
MASFPSSPLR IPGGNIYISP LKSPYKISEG LPTPTKMTPR SRILVSIGES FGTSEKFQKI NQMVCNSDRV LKRSAEGSNP PKPLKKLRFD IEGSDEADGS KHLPGESKFQ QKLAEMTSTR TRMQKQKMND SMDTSNKEEK HHHHHH |
| Purity |
> 95 % by SDS-PAGE and HPLC analyses. |
| Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin |
Less than 1 EU/μg of rHuRb137, His as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Usage |
This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
| Reference |
1. Lee WH, Shew JY, Hong FD, et al. 1987. Nature, 329: 642-5.
2. Lee WH, Bookstein R, Hong F, et al. 1987. Science, 235: 1394-9.
3. Toguchida J, McGee TL, Paterson JC, et al. 1993. Genomics, 17: 535-43.
4. Fattaey AR, Helin K, Dembski MS, et al. 1993. Oncogene, 8: 3149-56. |
| Background |
Rb encoded by the RB1 gene in humans, is expressed by retina and belongs to the etinoblastoma-associated protein family. The hole protein consists of 928 a.a. and the rHuRb fragment occupies sequence of 792-929 a.a.. Rb is a key regulator of entry into cell division that acts as a tumor suppressor. It has many functions, for example, promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C, and acts as a transcription repressor of E2F1 target genes and so on. The rHuRb is the region that rich of modified residue like phosphothreonine and N6-acetyllysine. |
|
|
첨부파일
|
|
|
|
|
|
