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카테고리
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Others
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CAT.NO
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LGP-60-019
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PRODUCT
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Recombinant Human Nesfatin-1
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SIZE
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100ug, 500ug, 1mg
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PRICE
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KRW 285,000, 940,000, 1,560,000
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Technical Parameters
| Synonyms |
Nucleobindin 2 |
| Accession |
P80303 |
| GeneID |
4925 |
| Source |
Escherichia coli. |
| Molecular Weight |
Approximately 9.6 kDa, a single non-glycosylated polypeptide chain containing 82 amino acids. |
| Quantity |
100µg/500µg/1000µg |
| AA Sequence |
VPIDIDKTKV QNIHPVESAK IEPPDTGLYY DEYLKQVIDV LETDKHFREK LQKADIEEIK SGRLSKELDL VSHHVRTKLD EL |
| Purity |
> 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity |
Fully biologically active when compared to standard. The biological activity is tested by in vivo assay using healthy wild type male mice (C57BL/6J). |
| Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin |
Less than 1 EU/µg of rHuNesfatin-1 as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Usage |
This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
| SDS-PAGE |
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| Reference |
1. Yang M, Zhang Z, Wang C, et al. 2012. Diabetes, 61: 1959-68.
2. Xia ZF, Fritze DM, Li JY, et al. 2012. Am J Physiol Gastrointest Liver Physiol, 303: G570-7.
3. Cowley MAandGrove KL. 2006. Cell Metab, 4: 421-2.
4. Pan W, Hsuchou H, Kastin AJ. 2007. Peptides, 28: 2223-8. |
| Background |
Nesfatin is a metabolic polypeptide and is the N-terminal region of the precursor protein, Nucleobindin2 (encoded by NUCB2 gene). It is a naturally occurring protein and originally identified as a hypothalamic neuropeptide. Additionally, Nesfatin can be found in other areas of brain, and in pancreatic isletsβ-cells, gastric endocrine cells and adipocytes. It is responsible for regulating appetite and production of body fat. Excess nesfatin-1 in the brain leads to a loss of appetite, less frequent hunger, a 'sense of fullness', and a drop in body fat and weight. A lack of nesfatin-1 in the brain leads to an increase of appetite, more frequent episodes of hunger, an increase of body fat and weight, and the inability to 'feel full'. |
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첨부파일
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