Product name |
Bovine HSP70(Heat shock protein 70) ELISA Kit
|
Catalogue No. |
LEB0032
|
Alias |
DAAP-21F2.7 ELISA Kit, FLJ54328 ELISA Kit, HSP70-1B ELISA Kit, HSP70-2 ELISA Kit, HSPA1A ELISA Kit, heat shock 70kD protein 1B ELISA Kit
|
Detection method |
Sandwich ELISA, Double Antibody
|
Size |
96T
|
Range |
0.313-20ng/ml
|
Sensitivity |
< 0.188ng/ml
|
Standard curve |
|
Storage |
4 ¡É for 6 months
|
Recovery |
Matrices listed below were spiked with certain level of HSP70 and the recovery rates were calculated by comparing the measured value to the expected amount of HSP70 in samples.
Matrix |
Recovery range(%) |
Average(%) |
serum(n=5) |
86-102 |
95 |
EDTA plasma(n=5) |
85-105 |
97 |
heparin plasma(n=5) |
90-105 |
96 | |
Linearity |
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HSP70 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample |
1:2 |
1:4 |
1:8 |
1:16 |
serum(n=5) |
96-102% |
86-97% |
93-102% |
86-104% |
EDTA plasma(n=5) |
87-97% |
83-96% |
84-95% |
82-98% |
heparin plasma(n=5) |
84-91% |
86-93% |
80-94% |
81-88% | |
CV(%) |
Intra-Assay: CV<8% Inter-Assay: CV<10%
|
Note |
For Research Use Only
|